Exploring the genetic diversity of Tylodelphys (Diesing, 1850) metacercariae in the cranial and body cavities of Mexican freshwater fishes using nuclear and mitochondrial DNA sequences, with the description of a new species
Por:
Sereno-Uribe, Ana L., Andrade-Gomez, Leopoldo, de León G.P.P., Garcia-Varela, Martin
Publicada:
1 ene 2019
Ahead of Print:
1 ene 2018
Resumen:
Members of the genus Tylodelphys Diesing, 1850 are endoparasites of
fish-eating birds, particularly ciconiids, anhingids, and podicipedids
across the globe. Metacercariae of Tylodelphys spp. were collected from
the cranial and body cavities of freshwater fishes in central and
northern Mexico; adults were recovered from the intestine of two species
of freshwater diving birds of the family Podicipedidae, commonly known
as grebes, in two locations of central Mexico. Specimens were sequenced
for two molecular markers, the internal transcribed spacers (ITS1 and
ITS2) plus 5.8S gene of the nuclear ribosomal DNA and of the cytochrome
c oxidase subunit 1 from mitochondrial DNA. The genetic divergence among
the 25 samples (16 metacercariae and 9 adults) and between the newly
sequenced specimens and those deposited in the GenBank were estimated.
Maximum likelihood and Bayesian inference analyses inferred with each
data set revealed the existence of five genetic lineages. Eight
metacercariae analyzed in this study were nested in two divergent
lineages previously recognized as Tylodelphys sp. 5 and Tylodelphys sp.
6 (sensu Locke et al., Int J Parasitol, 45:841-855, 2015). Five adult
specimens recovered from the intestine of the least grebe (Tachybaptus
dominicus Linnaeus, 1766) in Tecocomulco Lake, Hidalgo State, nested in
a single clade with other sequences identified previously as Tylodelphys
aztecae, expanding its distribution range in other areas of central
Mexico. The isolates of the metacercariae found in the cranial cavity of
the shortfin silverside, Chirostoma humboldtianum Valenciennes, 1835
from Zacapu Lake in central Mexico formed a monophyletic lineage and
were recognized as an undescribed species of Tylodelphys. The lack of
adult specimens of this lineage in our samples prevented a formal
description. However, the metacercariae collected in the cranial cavity
of the silverside, Chirostoma jordani Woolman, 1894 and the adult
specimens recovered from the intestine of the western grebe,
Aechmophorus occidentalis (Lawrence, 1858) from Cuitzeo Lake formed a
monophyletic clade, allowing us to link both stages of the life cycle
and to describe this as a new species, Tylodelphys kuerepus n. sp. The
new species represents the eighth species of the genus described in the
Americas and the fourth in the Nearctic region. We briefly discuss the
ecological associations between the metacercariae and their second
intermediate hosts in relation to the genetic diversity patterns
uncovered in our study.
Filiaciones:
Sereno-Uribe, Ana L.:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Mexico, Distrito Federal 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ave Univ 3000, Mexico City 04510, DF, Mexico
Andrade-Gomez, Leopoldo:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Mexico, Distrito Federal 04510, Mexico
Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Mexico, Distrito Federal 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ave Univ 3000, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Ciencias Biol, Ciudad Univ,Ave Univ 3000, Mexico City 04510, DF, Mexico
de León G.P.P.:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Mexico, Distrito Federal 04510, Mexico
Garcia-Varela, Martin:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Avenida Universidad 3000, Mexico, Distrito Federal 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ave Univ 3000, Mexico City 04510, DF, Mexico
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