DNA Synthesis Is Activated in Mosquitoes and Human Monocytes During the Induction of Innate Immune Memory
Por:
Cime-Castillo J., Arts R.J.W., De León V.V.-P., Moreno-Torres R., Hernández-Martínez S., Recio-Totoro B., Claudio-Piedras F., Netea M.G., Lanz-Mendoza H.
Publicada:
30 nov 2018
Resumen:
Endoreplication is a cell cycle program in which cells replicate their
genomes without undergoing mitosis and cytokinesis. For the normal
development of many organisms (from fungi to humans) and the formation
of their organs, endoreplication is indispensable. The aim of the
present study was to explore whether endoreplication and DNA synthesis
are relevant processes during the induction of trained innate immunity
in human monocytes and in the Anopheles albimanus mosquito cell line.
During the induction of trained immunity in both models, endoreplication
markers were overexpressed and we observed an increase in DNA synthesis
with an augmented copy number of genes essential for trained immunity.
Blocking DNA synthesis prevented trained immunity from being
established. Overall, these findings suggest that DNA synthesis and
endoreplication are important mechanisms involved in inducing innate
immune memory. They have probably been conserved throughout evolution
from invertebrates to humans.
Filiaciones:
Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Mexico
Department of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands
Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Mexico City, Mexico
Department for Genomics and Immunoregulation, Life and Medical Sciences Institute, University of Bonn, Bonn, Germany
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