Sex-Associated Differential mRNA Expression of Cytokines and Its Regulation by Sex Steroids in Different Brain Regions in a Plasmodium berghei ANKA Model of Cerebral Malaria
Por:
Legorreta-Herrera M., Nava-Castro K.E., Palacios-Arreola M.I., Hernández-Cervantes R., Aguilar-Castro J., Cervantes-Candelas L.A., Morales-Montor J.
Publicada:
1 ene 2018
Resumen:
Cerebral malaria (CM) is the major complication associated with death in
malaria patients, and its pathogenesis is associated with excessive
proinflammatory cytokine production. Notably, the severity and mortality
of natural infections with Plasmodium are higher in males than females,
suggesting that sexual hormones influence both the pathogenesis of and
immune response in CM. However, no studies on inflammation mediators in
the brains of both sexes have been reported. In this work, the mRNA
expression levels of the proinflammatory cytokines IL-1 beta, IFN-gamma,
TNF-alpha, and IL-2 were measured in the preoptic area, hypothalamus,
hippocampus, olfactory bulb, frontal cortex, and lateral cortex regions
of gonadectomized female and male CBA/Ca mice infected with P. berghei
ANKA (a recognized experimental CM model). Our findings demonstrate that
both infection with P. berghei ANKA and gonadectomy trigger a cerebral
sex dimorphic mRNA expression pattern of the cytokines IL-1 beta,
TNF-alpha, IFN-gamma, and IL-2. This dimorphic cytokine pattern was
different in each brain region analysed. In most cases, infected males
exhibited higher mRNA expression levels than females, suggesting that
sexual hormones differentially regulate the mRNA expression of
proinflammatory cytokines in the brain and the potential use of gonadal
steroids or their derivates in the immunomodulation of cerebral malaria.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Inmunol Mol, Ciudad De Mexico 09230, Mexico
Univ Nacl Autonoma Mexico, Dept Ciencias Ambientales, Ctr Ciencias Atmosfera, Lab Genotoxicol & Med Ambientales, Ciudad De Mexico 04510, Mexico
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, AP 70228, Ciudad De Mexico 04510, Mexico
Laboratorio de Inmunología Molecular, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, Ciudad de México, 09230, Mexico
Laboratorio de Genotoxicología y Medicina Ambientales, Departamento de Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, AP 70228, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
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