Sodium caseinate and alpha-casein inhibit proliferation of the mouse myeloid cell line 32D cl3 via TNF-alpha
Por:
Weiss-Steider, Benny, Cordova, Yolanda, Aguiniga-Sanchez, Itzen, Ledesma-Martinez, Edgar, Dominguez-Melendez, Vanihamin, Santiago-Osorio, Edelmiro
Publicada:
1 jun 2019
Resumen:
Introduction: Sodium caseinate (CS) and its components (alpha-casein,
beta-casein and kappa-casein) have been shown to inhibit the
proliferation of the mouse hematopoietic 32D cl3 cell line and induce
its differentiation into macrophages. It is known that alpha-casein
induce lL-1 beta production and this cytokine inhibits the proliferation
by production of tumor necrosis factor alpha (TNF-alpha), but it is not
known if CS and the caseins inhibit the proliferation by TNF-alpha
production. Objective: Evaluate if CS and alpha-casein, beta-casein and
kappa-casein inhibit the proliferation on 32D cl3 cell line via
TNF-alpha.
Objective: Evaluate if CS and alpha-casein, beta-casein and kappa-casein
inhibit the proliferation on 32D cl3 cell line via TNF-alpha.
Materials and methods: To evaluate cell proliferation, different
concentrations of CS, alpha-casein, beta-casein and kappa-casein were
used on 32D cl3 cells. Cell viability was assessed by MTT, induction to
apoptosis by flow cytometry and TNF-alpha synthesis by ELISA.
Additionally, anti-TNF-alpha neutralization assays were performed on 32D
cells treated with CS and alpha-casein and proliferation was evaluated.
Results: The results show that CS, alpha-casein, beta-casein and
kappa-casein reduce proliferation of the 32D cl3 cell line, without
effect on viability, and only CS and alpha-casein induce apoptosis and
release of TNF-alpha. The 32D cl3 cells treated with CS and alpha-casein
reestablished their proliferation by using antibodies anti-TNF-alpha.
Conclusion: TNF-alpha is principally responsible for the inhibition of
proliferation in 32D cl3 cells treated with CS or alpha-casein.
Filiaciones:
Weiss-Steider, Benny:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Cordova, Yolanda:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Aguiniga-Sanchez, Itzen:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Ledesma-Martinez, Edgar:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Dominguez-Melendez, Vanihamin:
Univ Veracruzana, Ctr Estudios & Serv Salud, Lab Biol Celular & Mol, Xalapa, Veracruz, Mexico
Santiago-Osorio, Edelmiro:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
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