Molecular data reveal high diversity of Uvulifer (Trematoda: Diplostomidae) in Middle America, with the description of a new species
Por:
Lopez-Jimenez, A., Perez-Ponce de Leon, G., Garcia-Varela, M.
Publicada:
1 nov 2018
Resumen:
Members of the genus Uvulifer are distributed worldwide and infect
aquatic snails and freshwater fishes as first and second intermediate
hosts, respectively, and fish-eating birds (kingfishers) as definitive
hosts. Metacercariae of Uvulifer spp. were collected from the fins and
skin of 20 species of freshwater fishes in Mexico, Guatemala, Honduras,
Nicaragua and Costa Rica, and the adults were recovered from the
intestine of kingfishers in four localities of Mexico. The genetic
divergence among 76 samples (64 metacercariae and 12 adults) was
estimated by sequencing the 28S and 5.8S nuclear genes, as well as the
internal transcribed spacers ITS1 and ITS2, and one mitochondrial gene
(cox1). Maximum likelihood and Bayesian inference analyses inferred with
each dataset showed a high genetic diversity within the genus Uvulifer
across Middle America, revealing the existence of four genetic lineages
that exhibit some level of host specificity to their second intermediate
hosts. The metacercariae of lineage 1 were associated with characids and
cyprinids in central and northern Mexico. Metacercariae of lineages 2
and 3 were associated with cichlids distributed widely across Middle
America. The lack of adults of these lineages in kingfishers, in
lineages 2 and 3, or the fact that just a few adult specimens were
recovered, as in lineage 1, prevented a formal description of these
species. The metacercariae of lineage 4 were found in poeciliids, across
a distribution range comprising Mexico, Guatemala, Honduras and
Nicaragua, and the adult was found in the green kingfisher in Mexico.
The number of specimens sampled for lineage 4, for both gravid adults
and metacercariae, allowed us to describe a new species, Uvulifer
spinatus n. sp. We describe the new species herein and we discuss
briefly the genetic diversity in Uvulifer spp. and the importance of
using DNA sequences to properly characterize parasite diversity.
Filiaciones:
Lopez-Jimenez, A.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Perez-Ponce de Leon, G.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Garcia-Varela, M.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
|