Bacterial and archaeal profiling of hypersaline microbial mats and endoevaporites, under natural conditions and methanogenic microcosm experiments
Por:
Garcia-Maldonado, Jose Q., Escobar-Zepeda, Alejandra, Raggi, Luciana, Bebout, Brad M., Sanchez-Flores, Alejandro, Lopez-Cortes, Alejandro
Publicada:
1 nov 2018
Resumen:
Bacterial and archaeal community structure of five microbial
communities, developing at different salinities in Baja California Sur,
Mexico, were characterized by 16S rRNA sequencing. The response of the
microbial community to artificial changes in salinity-sulfate
concentrations and to addition of trimethylamine was also evaluated in
microcosm experiments. Ordination analyses of the microbial community
structure showed that microbial composition was distinctive for each
hypersaline site. Members of bacteria were dominated by Bacteroidetes
and Proteobacteria phyla, while Halobacteria of the Euryarchaeota phylum
was the most represented class of archaea for all the environmental
samples. At a higher phylogenetic resolution, methanogenic communities
were dominated by members of the Methanosarcinales, Methanobacteriales
and Methanococcales orders. Incubation experiments showed that putative
hydrogenotrophic methanogens of the Methanomicrobiales increased in
abundance only under lowest salinity and sulfate concentrations.
Trimethylamine addition effectively increased the abundance of
methylotrophic members from the Methanosarcinales, but also increased
the relative abundance of the Thermoplasmata class, suggesting the
potential capability of these microorganisms to use trimethylamine in
hypersaline environments. These results contribute to the knowledge of
microbial diversity in hypersaline environments from Baja California
Sur, Mexico, and expand upon the available information for uncultured
methanogenic archaea in these ecosystems.
Filiaciones:
Inst Politecn Nacl, CONACYT Ctr Invest & Estudios Avanzados, Unidad Merida, Antigua Carretera Progreso Km 6, Yucatan 97310, Mexico
Univ Nacl Autonoma Mexico, Inst Biotechnol, Unidad Univ Secuenciac Mas & Bioinformat, Mexico City, DF, Mexico
Univ Michoacana, CONACYT Inst Invest Agr & Forest, Morelia, Michoacan, Mexico
NASA, Exobiol Branch, Ames Res Ctr, Moffett Field, CA USA
Inst Politecn Nacl 195, Ctr Invest Biol Noroeste, Lab Geomicrobiol & Biotecnol, La Paz 23096, Baja California, Mexico
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