Assessment of the effective dose of an experimental intramuscular formulation against immature and adult Fasciola hepatica in sheep
Por:
Ibarra-Velarde, F., Vera-Montenegro, Y., Flores-Ramos, M., Canto-Alarcon, G. J., Hernandez-Campos, A., Alcala-Canto, Yazmin, Castillo, R.
Publicada:
30 ago 2018
Resumen:
The effective dose of an injectable prodrug, named compound alpha prodrug, against immature and adult Fasciola hepatica in experimentally infected sheep was determined. In a first experiment, 30 sheep were infected with Fasciola hepatica on day 0 and 50. After microscopic detection of faecal eggs on day 80, groups (n = 6) 1 to 3 were treated with 6, 8 and 10 mg/kg of the experimental water-soluble prodrug compound alpha intramuscularly, respectively. Group 4 was treated with closantel and group 5 remained untreated. Copromicroscopical examinations were made on day 0, 80 and 108. On day 110, trematodes were collected from the bile ducts. Fasciolicide efficacy was assessed as a percentage of fluke-egg and adult-fluke reduction. Fluke length was also recorded. In a second experiment aimed to assess the fasciolicide activity of compound alpha prodrug against four-week-old flukes, 12 sheep were infected on day 0 and allocated into two groups (n = 6). On day 50 post infection, group A was treated with the experimental water-soluble prodrug compound alpha at 6 mg/kg/IM and B remained untreated. Fasciolicide activity was assessed on day 80 after collection, microscopic observation and measurement of flukes present in the parenchyma for immature stages and on day 108 for adults. Egg output decreased 91.2, 96.0, 98.8 and 94.9% for groups 1, 2, 3 and 4, respectively. Compound alpha prodrug cleared 97.6%, 98.51% and 100% of adult stages in a dose-dependent manner. Closantel killed 81.95% flukes. Regarding the second experiment, 81.2% efficacy was achieved. Immature flukes were significantly smaller in the treated group. It is concluded that the intramuscular application of compound alpha prodrug exerted fasciolicide efficacy against adults of Fasciola hepatica. © 2018 Elsevier B.V.
Filiaciones:
Ibarra-Velarde, F.:
Facultad de Medicina Veterinaria y Zootecnia, Departamento de Parasitología, UNAM, México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Parasitol, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico
Vera-Montenegro, Y.:
Facultad de Medicina Veterinaria y Zootecnia, Departamento de Parasitología, UNAM, México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Parasitol, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico
Flores-Ramos, M.:
Facultad de Medicina Veterinaria y Zootecnia, Departamento de Parasitología, UNAM, México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Parasitol, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico
Canto-Alarcon, G. J.:
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Qro. CP., Mexico
Univ Autonoma Queretaro, Fac Ciencias Nat, Queretaro 76230, Mexico
Hernandez-Campos, A.:
Facultad de Química, Departamento de Farmacia, UNAM, México City, Mexico
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Mexico City 04510, DF, Mexico
Alcala-Canto, Yazmin:
Facultad de Medicina Veterinaria y Zootecnia, Departamento de Parasitología, UNAM, México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Parasitol, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico
Castillo, R.:
Facultad de Química, Departamento de Farmacia, UNAM, México City, Mexico
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Mexico City 04510, DF, Mexico
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