Phylogenetic analysis and characterization of the complete hepatitis E virus genome (Zoonotic genotype 3) in swine samples from Mexico
Por:
Sotomayor-Gonzalez, Alicia, Trujillo-Ortega, Maria E., Taboada-Ramirez, Blanca I., Sandoval-Jaime, Carlos, Sarmiento-Silva, Rosa E.
Publicada:
1 ago 2018
Resumen:
Hepatitis E virus (HEV) is an emerging public health problem with an estimated 20 million infections each year. In Mexico, Orthohepevirus A, genotype 2, has been reported in humans, but genotype 3 has only been reported in swine (zoonotic). No diagnostic tests are publicly available in Mexico, and only partial sequences have been reported from swine samples. Hence, research is necessary to determine circulating strains, understand the features and dynamics of infection on pig farms, determine how to implement surveillance programs, and to assess public health risks. In this study, a next-generation sequencing (NGS) approach was applied to obtain a complete genome of swine HEV. Liver, feces, and bile samples were taken at slaughterhouses and a farm in Mexico. RT-PCR was used to determine positive samples and confirmed by Sanger sequencing. Of the 64 slaughterhouse samples, one bile sample was positive (B1r) (1.56%). Of 21 sample pools from farm animals, 14 were positive (66.66%), representing all stages of production. A complete sequence strain MXCDg3_B1c|_2016 was obtained from the bile of a domestic swine in the fattening stage. In addition, two partial sequences—MXCDg3_H2cons|_2016 (1473 nt) and MXCDg3_C3Acons|_2016 (4777 nt)—were obtained from sampled farm animals. Comparison with all reported genome HEV sequences showed similarity to genotype 3 subgenotype a (G3a), which has been previously reported in acute cases of human hepatitis in the US, Colombia, China, and Japan. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Laboratory of Virology, Microbiology and Immunology Department, Veterinary Medicine and Husbandry Faculty, National Autonomous University of Mexico (UNAM), Mexico City, Mexico
Academic Council of the Biological, Chemical and Medical Sciences, National Autonomous University of Mexico (UNAM), Mexico City, Mexico
Swine Medicine and Husbandry Department, Veterinary Medicine and Husbandry Faculty, National Autonomous University of Mexico (UNAM), Mexico City, Mexico
Biotechnology Institute (IBT), National Autonomous University of Mexico (UNAM), Cuernavaca, Morelos, Mexico
Natl Autonomous Univ Mexico UNAM, Vet Med & Husb Fac, Microbiol & Immunol Dept, Lab Virol, Mexico City 04510, DF, Mexico
Natl Autonomous Univ Mexico UNAM, Acad Council Biol Chem & Med Sci, Mexico City 04510, DF, Mexico
Natl Autonomous Univ Mexico UNAM, Vet Med & Husb Fac, Swine Med & Husb Dept, Mexico City 04510, DF, Mexico
Natl Autonomous Univ Mexico UNAM, Biotechnol Inst IBT, Cuernavaca 62209, Morelos, Mexico
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