Ultrastructural damage in Streptococcus mutans incubated with saliva and histatin 5
Por:
Fernandez-Presas, A. M., Marquez Torres, Y., Garcia Gonzalez, R., Reyes Torres, A., Becker Fauser, I., Rodriguez Barrera, H., Ruiz Garcia, B., Toloza Medina, R., Delgado Dominguez, J., Molinari Soriano, J. L.
Publicada:
1 mar 2018
Resumen:
Objective: To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. Methods: S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. Ultrastructural damage was evaluated by transmission electron microscopy. Ultrastructural localization of histatin 5 was examined using immunogold labeling. Apoptotic cell death was determined by flow cytometry (TUNEL). Results: A decrease in the bacteria numbers was observed after incubation with saliva, saliva with histatin 5 or histatin 5 compared to the control group (p<0.0001). Ultrastructural damage in S. mutans incubated with saliva was found in the cell wall. Saliva plus histatin 5 induced a cytoplasmic granular pattern and decreased the distance between the plasma membrane bilayers, also found after incubation with histatin 5, together with pyknotic nucleoids. Histatin 5 was localized on the bacterial cell walls, plasma membranes, cytoplasm and nucleoids. Apoptosis was found in the bacteria incubated with saliva (63.9%), saliva plus histatin 5 (71.4%) and histatin 5 (29.3%). Apoptosis in the control bacteria was 0.2%. Conclusions: Antibacterial activity against S. mutans and the morphological description of damage induced by saliva and histatin 5 was demonstrated. Pyknotic nucleoids observed in S. mutans exposed to saliva, saliva plus histatin 5 and histatin 5 could be an apoptosis-like death mechanism. The knowledge of the damage generated by histatin 5 and its intracellular localization could favor the design of an ideal peptide as a therapeutic agent. © 2018 Elsevier Ltd
Filiaciones:
Fernandez-Presas, A. M.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Marquez Torres, Y.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Garcia Gonzalez, R.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Reyes Torres, A.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Becker Fauser, I.:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Hosp Gen Mexico, 148 Col Doctores, Mexico City 06726, DF, Mexico
Rodriguez Barrera, H.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Ruiz Garcia, B.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Toloza Medina, R.:
Fac Med, Dept Microbiol & Parasitol, 1er Piso,Edificio A Invest, Av Univ 3000, Mexico City 04510, DF, Mexico
Delgado Dominguez, J.:
Univ Nacl Autonoma Mexico, Unidad Invest Med Expt, Hosp Gen Mexico, 148 Col Doctores, Mexico City 06726, DF, Mexico
Molinari Soriano, J. L.:
Inst Fisiol Celular, Dept Bioquim & Biol Estructurol, Av Univ 3000, Mexico City 04510, DF, Mexico
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