A novel progesterone receptor membrane component (PGRMC) in the human and swine parasite Taenia solium: Implications to the host-parasite relationship
Por:
Aguilar-Díaz H., Nava-Castro K.E., Escobedo G., Domínguez-Ramírez L., García-Varela M., Del Río-Araiza V.H., Palacios-Arreola M.I., Morales-Montor J.
Publicada:
9 mar 2018
Resumen:
Background: We have previously reported that progesterone (P4) has a direct in vitro effect on the scolex evagination and growth of Taenia solium cysticerci. Here, we explored the hypothesis that the P4 direct effect on T. solium might be mediated by a novel steroid-binding parasite protein. Methods: By way of using immunofluorescent confocal microscopy, flow cytometry analysis, double-dimension electrophoresis analysis, and sequencing the corresponding protein spot, we detected a novel PGRMC in T. solium. Molecular modeling studies accompanied by computer docking using the sequenced protein, together with phylogenetic analysis and sequence alignment clearly demonstrated that T. solium PGRMC is from parasite origin. Results: Our results show that P4 in vitro increases parasite evagination and scolex size. Using immunofluorescent confocal microscopy, we detected that parasite cells showed expression of a P4-binding like protein exclusively located at the cysticercus subtegumental tissue. Presence of the P4-binding protein in cyst cells was also confirmed by flow cytometry. Double-dimension electrophoresis analysis, followed by sequencing the corresponding protein spot, revealed a protein that was previously reported in the T. solium genome belonging to a membrane-associated progesterone receptor component (PGRMC). Molecular modeling studies accompanied by computer docking using the sequenced protein showed that PGRMC is potentially able to bind steroid hormones such as progesterone, estradiol, testosterone and dihydrodrotestosterone with different affinities. Phylogenetic analysis and sequence alignment clearly demonstrated that T. solium PGRMC is related to a steroid-binding protein of Echinoccocus granulosus, both of them being nested within a cluster including similar proteins present in platyhelminths such as Schistocephalus solidus and Schistosoma haematobium. Conclusion: Progesterone may directly act upon T. solium cysticerci probably by binding to PGRMC. This research has implications in the field of host-parasite co-evolution as well as the sex-associated susceptibility to this infection. In a more practical matter, present results may contribute to the molecular design of new drugs with anti-parasite actions. © 2018 The Author(s).
Filiaciones:
Aguilar-Díaz H.:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias INIFAP, Jiutepec, Morelos, Mexico
INIFAP, Ctr Nacl Invest Disciplinaria Parasitol Vet, Jiutepec 62550, Morelos, Mexico
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias INIFAP, Jiutepec, Morelos, CP 62550, Mexico
Nava-Castro K.E.:
Laboratorio de Genotoxicología y Medicina Ambientales, Departamento De.Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad de Mexico, Mexico
Univ Nacl Autonoma Mexico, Lab Genotoxicol & Med Ambientales, Dept Ciencias Ambientales, Ctr Ciencias Atmosfera, Mexico City 04510, DF, Mexico
Laboratorio de Genotoxicología y Medicina Ambientales, Departamento De.Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad de Mexico, 04510, Mexico
Escobedo G.:
Unidad de Medicina Experimental, Hospital General de México Dr. Eduardo Liceaga, México DF, Mexico
Hosp Gen Mexico Dr Eduardo Liceaga, Unidad Med Expt, Mexico City 06726, DF, Mexico
Unidad de Medicina Experimental, Hospital General de México Dr. Eduardo Liceaga, México DF, 06726, Mexico
Domínguez-Ramírez L.:
Departamento de Ciencias Químico-Biológicas, Universidad de Las Américas Puebla, Sta. Catarina Mártir, Cholula, Puebla, Mexico
Univ Americas Puebla, Dept Ciencias Quim Biol, Cholula 72810, Mexico
Departamento de Ciencias Químico-Biológicas, Universidad de Las Américas Puebla, Sta. Catarina Mártir, Cholula, Puebla, C.P 72810, Mexico
García-Varela M.:
Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de Mexico, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico
Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de Mexico, DF, CP 04510, Mexico
Del Río-Araiza V.H.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de Mexico, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, AP 70228, Mexico City 04510, DF, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de Mexico, DF, 04510, Mexico
Palacios-Arreola M.I.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de Mexico, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, AP 70228, Mexico City 04510, DF, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de Mexico, DF, 04510, Mexico
Morales-Montor J.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de Mexico, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, AP 70228, Mexico City 04510, DF, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de Mexico, DF, 04510, Mexico
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