Characterization of the Symbiotic Nitrogen-Fixing Common Bean Low Phytic Acid (lpa1) Mutant Response to Water Stress
Por:
Chiozzotto R., Ramírez M., Talbi C., Cominelli E., Girard L., Sparvoli F., Hernández G.
Publicada:
1 feb 2018
Resumen:
The common bean (Phaseolus vulgaris L.) low phytic acid (lpa1)
biofortified genotype produces seeds with improved nutritional
characteristics and does not display negative pleiotropic effects. Here
we demonstrated that lpa1 plants establish an efficient nitrogen-fixing
symbiosis with Rhizobium etli CE3. The lpa1 nodules showed a higher
expression of nodule-function related genes than the nodules of the
parental wild type genotype (BAT 93). We analyzed the response to water
stress of lpa1 vs. BAT 93 plants grown under fertilized or under
symbiotic N-2-fixation conditions. Water stress was induced by water
withholding (up to 14% soil moisture) to fertilized or R. etli
nodulated plants previously grown with normal irrigation. The fertilized
lpa1 plants showed milder water stress symptoms during the water
deployment period and after the rehydration recovery period when lpa1
plants showed less biomass reduction. The symbiotic water-stressed lpa1
plants showed decreased nitrogenase activity that coincides with
decreased sucrose synthase gene expression in nodules; lower turgor
weight to dry weight (DW) ratio, which has been associated with higher
drought resistance index; downregulation of carbon/nitrogen
(C/N)-related and upregulation of stress-related genes. Higher
expression of stress-related genes was also observed in bacteroids of
stressed lpa1 plants that also displayed very high expression of the
symbiotic cbb(3) oxidase (fixNd).
Filiaciones:
Chiozzotto R.:
Center for Genomic Sciences, National Autonomous University of Mexico, Av, Universidad 1001, Cuernavaca, Mor., Mexico
Ramírez M.:
Center for Genomic Sciences, National Autonomous University of Mexico, Av, Universidad 1001, Cuernavaca, Mor., Mexico
Talbi C.:
Center for Genomic Sciences, National Autonomous University of Mexico, Av, Universidad 1001, Cuernavaca, Mor., Mexico
Cominelli E.:
Institute of Agricultural Biology and Biotechnology, National Research Council, IBBA-CNR, Via Edoardo Bassini 15, Milano, Italy
Girard L.:
Center for Genomic Sciences, National Autonomous University of Mexico, Av, Universidad 1001, Cuernavaca, Mor., Mexico
Sparvoli F.:
Institute of Agricultural Biology and Biotechnology, National Research Council, IBBA-CNR, Via Edoardo Bassini 15, Milano, Italy
Hernández G.:
Center for Genomic Sciences, National Autonomous University of Mexico, Av, Universidad 1001, Cuernavaca, Mor., Mexico
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