Identification and characterization of Taenia solium enolase as a plasminogen-binding protein
Por:
Ayón-Núñez D.A., Fragoso G., Espitia C., García-Varela M., Soberón X., Rosas G., Laclette J.P., Bobes R.J.
Publicada:
1 jun 2018
Resumen:
The larval stage of Taenia solium (cysticerci) is the causal agent of human and swine cysticercosis. When ingested by the host, T. solium eggs are activated and hatch in the intestine, releasing oncospheres that migrate to various tissues and evolve into cysticerci. Plasminogen (Plg) receptor proteins have been reported to play a role in migration processes for several pathogens. This work is aimed to identify Plg-binding proteins in T. solium cysticerci and determine whether T. solium recombinant enolase (rTsEnoA) is capable of specifically binding and activating human Plg. To identify Plg-binding proteins, a 2D-SDS-PAGE ligand blotting was performed, and recognized spots were identified by MS/MS. Seven proteins from T. solium cysticerci were found capable of binding Plg: fascicilin-1, fasciclin-2, enolase, MAPK, annexin, actin, and cytosolic malate dehydrogenase. To determine whether rTsEnoA binds human Plg, a ligand blotting was performed and the results were confirmed by ELISA both in the presence and absence of eACA, a competitive Plg inhibitor. Finally, rTsEnoA-bound Plg was activated to plasmin in the presence of tPA. To better understand the evolution of enolase isoforms in T. solium, a phylogenetic inference analysis including 75 enolase amino acid sequences was conducted. The origin of flatworm enolase isoforms, except for Eno4, is independent of their vertebrate counterparts. Therefore, herein we propose to designate tapeworm protein isoforms as A, B, C, and 4. In conclusion, recombinant enolase showed a strong plasminogen binding and activating activity in vitro. T. solium enolase could play a role in parasite invasion along with other plasminogen-binding proteins. © 2018 Elsevier B.V.
Filiaciones:
Ayón-Núñez D.A.:
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico
Fragoso G.:
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico
Espitia C.:
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico
García-Varela M.:
Dept. of Zoology, Institute of Biology, Universidad Nacional Autónoma de México, Mexico City, Mexico
Soberón X.:
Instituto Nacional de Medicina Genómica, Mexico City, Mexico
Rosas G.:
School of Medicine, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico
Laclette J.P.:
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico
Bobes R.J.:
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico
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