De novo assembly and transcriptome characterization of the freshwater prawn Palaemonetes argentinus: Implications for a detoxification response
Por:
García C.F., Pedrini N., Sánchez-Paz A., Reyna-Blanco C.S., Lavarias S., Muhlia-Almazán A., Fernández-Giménez A., Laino A., de-la-Re-Vega E., Lukaszewicz G., López-Zavala A.A., Brieba L.G., Criscitello M.F., Carrasco-Miranda J.S., García-Orozco K.D., Ochoa-Leyva A., Rudiño-Piñera E., Sanchez-Flores A., Sotelo-Mundo R.R.
Publicada:
1 feb 2018
Resumen:
Palaemonetes argentinus, an abundant freshwater prawn species in the northern and central region of Argentina, has been used as a bioindicator of environmental pollutants as it displays a very high sensitivity to pollutants exposure. Despite their extraordinary ecological relevance, a lack of genomic information has hindered a more thorough understanding of the molecular mechanisms potentially involved in detoxification processes of this species. Thus, transcriptomic profiling studies represent a promising approach to overcome the limitations imposed by the lack of extensive genomic resources for P. argentinus, and may improve the understanding of its physiological and molecular response triggered by pollutants. This work represents the first comprehensive transcriptome-based characterization of the non-model species P. argentinus to generate functional genomic annotations and provides valuable resources for future genetic studies. Trinity de novo assembly consisted of 24,738 transcripts with high representation of detoxification (phase I and II), anti-oxidation, osmoregulation pathways and DNA replication and bioenergetics. This crustacean transcriptome provides valuable molecular information about detoxification and biochemical processes that could be applied as biomarkers in further ecotoxicology studies. © 2017 Elsevier B.V.
Filiaciones:
Instituto de Investigaciones Bioquímicas de La Plata Dr. Rodolfo Brenner (INIBIOLP), CCT-La Plata CONICET-UNLP, Calle 60 y 120, La Plata 1900, Argentina
Laboratorio de Referencia, Análisis y Diagnóstico en Sanidad Acuícola, Centro de Investigaciones Biológicas del Noroeste S. C. (CIBNOR), Calle Hermosa 101. Col. Los Ángeles, 83106 Hermosillo, Sonora, Mexico
Departamentos de Microbiología Molecular, Medicina Molecular y Bioprocesos, Unidad Universitaria de Secuenciacián Masiva y Bioinformática, Instituto de Biotecnología (IBT), Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca 62210, Mexico
Instituto de Limnología de La Plata Dr. Raúl A. Ringuelet (ILPLA), CONICET CCT La Plata-Universidad Nacional de La Plata (UNLP), Boulevard 120 y 62, La Plata 1900, Argentina
Laboratorio de Bioenergética, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a Ejido La Victoria, Km 0.6, Hermosillo, Sonora 83304, Mexico
Laboratorio de Fisiología de Organismos Acuáticos y Biotecnología, Instituto de Investigaciones Marinas y Costeras (IIMYC), Facultad de Ciencias Exactas y Naturales CONICET, Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata 7600, Argentina
Universidad de Sonora, Blvd. Colosio y Reforma s/n, Hermosillo, Sonora 83000, Mexico
Laboratorio de Ecotoxicología, Instituto de Investigaciones Marinas y Costeras (IIMYC), Facultad de Ciencias Exactas y Naturales, CONICET, Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata 7600, Argentina
Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843, United States
Laboratorio de Estructura Biomolecular, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD). Carretera a Ejido La Victoria Km 0.6, Apartado Postal 1735, Hermosillo, Sonora 83304, Mexico
Calbiotech. 1935 Cordell Court. El Cajon, CA 92020, USA
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