A Positive TGF-ß/c-KIT Feedback Loop Drives Tumor Progression in Advanced Primary Liver Cancer
Por:
Rojas A., Zhang P., Wang Y., Foo W.C., Muñoz N.M., Xiao L., Wang J., Gores G.J., Hung M.-C., Blechacz B.
Publicada:
1 ene 2016
Categoría:
Cancer Research
Resumen:
Hepatocellular carcinoma (HCC) is globally the second most common cause of cancer mortality. The majority of HCC patients are diagnosed at advanced stage disease for which no curative treatments exist. TGF-ß has been identified as a potential therapeutic target. However, the molecular mechanisms mediating its functional switch from a tumor suppressor to tumor promoter in HCC and its interactions with other signaling pathways are poorly understood. Here, we demonstrate an aberrant molecular network between the TGF-ß and c-KIT pathway that mediates the functional switch of TGF-ß to a driver of tumor progression in HCC. TGF-ß/SMAD2 signaling transcriptionally regulates expression of the c-KIT receptor ligand (stem cell factor [SCF]) with subsequent auto- and paracrine activation of c-KIT/JAK1/STAT3 signaling. SCF induces TGF-ß1 ligand expression via STAT3, thereby forming a positive feedback loop between TGF-ß/SMAD and SCF/c-KIT signaling. This network neutralizes TGF-ß–mediated cell cycle inhibition and induces tumor cell proliferation, epithelial-to-mesenchymal-transition, migration, and invasion. Disruption of this feedback loop inhibits TGF-ß tumor-promoting effects and restores its antiproliferative functions. Consistent with our in vitro data, we demonstrate SCF overexpression and its correlation to SMAD2 and STAT3 activation in human HCC tumors, advanced tumor-node-metastasis stages, and shorter survival. CONCLUSIONS: Canonical TGF-ß and c-KIT signaling forms a positive, tumor-promoting feedback loop. Disruption of this loop restores TGF-ß tumor suppressor function and provides the rationale for targeting the TGF-ß/SCF axis as a novel therapeutic strategy for HCC. © 2016 The Authors
Filiaciones:
Rojas A.:
Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Zhang P.:
Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Wang Y.:
Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Foo W.C.:
Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Muñoz N.M.:
Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Xiao L.:
Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Wang J.:
Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Gores G.J.:
Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, United States
Hung M.-C.:
Department of Molecular Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
Graduate Institute of Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung, Taiwan
Blechacz B.:
Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center, Houston, TX, United States
|