Preparation of samples for leaf architecture studies, a method for mounting cleared leaves
Por:
Vasco A., Thadeo M., Conover M., Daly D.C.
Publicada:
1 ene 2014
Resumen:
Premise of the study: Several recent waves of interest in leaf architecture have shown an expanding range of approaches and applications across a number of disciplines. Despite this increased interest, examination of existing archives of cleared and mounted leaves shows that current methods for mounting, in particular, yield unsatisfactory results and deterioration of samples over relatively short periods. Although techniques for clearing and staining leaves are numerous, published techniques for mounting leaves are scarce. Methods and Results: Here we present a complete protocol and recommendations for clearing, staining, and imaging leaves, and, most importantly, a method to permanently mount cleared leaves. Conclusions: The mounting protocol is faster than other methods, inexpensive, and straightforward; moreover, it yields clear and permanent samples that can easily be imaged, scanned, and stored. Specimens mounted with this method preserve well, with leaves that were mounted more than 35 years ago showing no signs of bubbling or discoloration. © 2014 Bossu et al. Published by the Botanical Society of America.
Filiaciones:
Vasco A.:
New York Botanical Garden, 2900 Southern Blvd, Bronx, NY, United States
Thadeo M.:
New York Botanical Garden, 2900 Southern Blvd, Bronx, NY, United States
Conover M.:
New York Botanical Garden, 2900 Southern Blvd, Bronx, NY, United States
Daly D.C.:
New York Botanical Garden, 2900 Southern Blvd, Bronx, NY, United States
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