Degree of pectin methyl esterification in endosperm cell walls is involved in embryo bending in Arabidopsis thaliana
Por:
Cruz-Valderrama, Jose E., Jimenez-Duran, Karina, Zuniga-Sanchez, Esther, Salazar-Iribe, Alexis, Marquez-Guzman, Judith, Gamboa-deBuen, Alicia
Publicada:
1 ene 2018
Resumen:
The endosperm is a transitory structure involved in proper embryo
elongation. The cell walls of mature seed endosperm are generally
composed of a uniform distribution of cellulose, unesterified
homo-galacturonans, and arabinans. Recent studies suggest that changes
in cell wall properties during endosperm development could be related to
embryo growth. The degree of methyl esterification of homogalacturonans
may be involved in this endosperm tissue remodelling. The relevance of
the degree of homogalacturonan methyl esterification during seed
development was determined by immunohistochemical analyses using a panel
of probes with specificity for homogalaturonans with different degrees
of methyl esterification. Low-esterified and un-esterified
homogalacturonans were abundant in endosperm cells during embryo bending
and were also detected in mature embryos. BIDXII (BDX) could be involved
in seed development, because bdx-1 mutants had misshapen embryos. The
methyl esterification pattern described for WT seeds was different
during bdx-1 seed development; un-esterified homogalacturonans were
scarcely present in the cell walls of endosperm in bending embryos and
mature seeds. Our results suggested that the degree of methyl
esterification of homogalacturonans in the endosperm cell wall may be
involved in proper embryo development. (C) 2017 Elsevier Inc. All rights
reserved.
Filiaciones:
Cruz-Valderrama, Jose E.:
Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad Univ, Mexico City 04510, DF, Mexico
Jimenez-Duran, Karina:
Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico
Zuniga-Sanchez, Esther:
Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico
Salazar-Iribe, Alexis:
Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad Univ, Mexico City 04510, DF, Mexico
Marquez-Guzman, Judith:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City 04510, DF, Mexico
Gamboa-deBuen, Alicia:
Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad Univ, Mexico City 04510, DF, Mexico
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