Naegleria fowleri immunization modifies lymphocytes and APC of nasal mucosa
Por:
Carrasco-Yepez M.M., Campos-Rodríguez R., Reséndiz-Albor A.A., Peña-Juárez C., Contis-Montes de Oca A., Arciniega-Martínez I.M., Bonilla-Lemus P., Rojas-Hernandez S.
Publicada:
1 mar 2018
Resumen:
We investigated whether intranasal immunization with amoebic lysates
plus cholera toxin modified the populations of T and B lymphocytes,
macrophages and dendritic cells by flow cytometry from nose-associated
lymphoid tissue (NALT), cervical lymph nodes (CN), nasal passages (NP)
and spleen (SP). In all immunized groups, the percentage of CD4 was
higher than CD8 cells. CD45 was increased in B cells from mice
immunized. We observed IgA antibody-forming cell (IgA-AFC) response,
mainly in NALT and NP. Macrophages from NP and CN expressed the highest
levels of CD80 and CD86 in N.fowleri lysates with either CT or CT alone
immunized mice, whereas dendritic cells expressed high levels of CD80
and CD86 in all compartment from immunized mice. These were lower than
those expressed by macrophages. Only in SP from CT-immunized mice, these
costimulatory molecules were increased. These results suggest that
N.fowleri and CT antigens are taking by APCs, and therefore, protective
immunity depends on interactions between APCs and T cells from NP and
CN. Consequently, CD4 cells stimulate the differentiation from B
lymphocytes to AFC IgA-positive; antibody that we previously found
interacting with trophozoites in the nasal lumen avoiding the N.fowleri
attachment to nasal epithelium.
Filiaciones:
Carrasco-Yepez M.M.:
Proyecto CyMA, Los Reyes Iztacala, UIICSE, UNAM FES Iztacala, Tlalnepantla, Mexico
Campos-Rodríguez R.:
Departamento de Bioquímica, Escuela Superior de Medicina, Laboratorio de Bioquímica, Instituto Politécnico Nacional, Mexico City, Mexico
Reséndiz-Albor A.A.:
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Laboratorio de Inmunobiología Molecular y Celular, Instituto Politécnico Nacional, Mexico City, Mexico
Peña-Juárez C.:
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Laboratorio de Inmunobiología Molecular y Celular, Instituto Politécnico Nacional, Mexico City, Mexico
Contis-Montes de Oca A.:
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Laboratorio de Inmunobiología Molecular y Celular, Instituto Politécnico Nacional, Mexico City, Mexico
Arciniega-Martínez I.M.:
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Laboratorio de Inmunobiología Molecular y Celular, Instituto Politécnico Nacional, Mexico City, Mexico
Bonilla-Lemus P.:
Proyecto CyMA, Los Reyes Iztacala, UIICSE, UNAM FES Iztacala, Tlalnepantla, Mexico
Rojas-Hernandez S.:
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Laboratorio de Inmunobiología Molecular y Celular, Instituto Politécnico Nacional, Mexico City, Mexico
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