Simultaneous quantification of four antiretroviral drugs in breast milk samples from HIV-positive women by an ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method
Por:
Ramirez-Ramirez, Alicia, Sanchez-Serrano, Elias, Loaiza-Flores, Giselle, Plazola-Camacho, Noemi, Georgina Rodriguez-Delgado, Rosa, Figueroa-Damian, Ricardo, Dominguez-Castro, Mauricio, Lopez-Martinez, Margarita, Flores-Garcia, Zayra, Hernandez-Pineda, Jessica
Publicada:
19 ene 2018
Resumen:
The primary strategy to avoid mother-to-child transmission of human
immunodeficiency virus (HIV) through breastfeeding is administration of
highly active antiretroviral therapy (HAART) to HIV-positive pregnant
women. Because significant changes in the pharmacokinetics of
antiretroviral (ARV) drugs occur during pregnancy, quantifying HAART and
the viral load in breast milk in this population is essential. Here, we
developed an analytical assay for the simultaneous quantification of
four ARV drugs in breast milk using ultra-performance liquid
chromatography coupled to tandem mass spectrometry. We validated this
method following Mexican and international guidelines. ARV drugs. We
extracted the ARV drugs from 200 mu L samples of breast milk and
detected these drugs in a triple quadrupole mass spectrometer with
positive electrospray ionization. The validated concentration ranges
(ng/mL) for zidovudine, lamivudine, lopinavir, and ritonavir were
12.5-750, 50-2500, 100-5000 and 5 to 250, respectively. Additionally,
the absolute recovery percentages (and matrix effects) were 91.4 (8.39),
88.78 (28.75), 91.38 (11.77) and 89.78 (12.37), respectively. We
determined that ARV drugs are stable for 24 h at 8 degrees C and 24
degrees C for 15 days at - 80 degrees C. This methodology had the
capacity for simultaneous detection; separation; and accurate, precise
quantification of ARV drugs in human breast milk samples according to
Mexican standard laws and United States Food and Drug Administration
guidelines.
Filiaciones:
Ramirez-Ramirez, Alicia:
Natl Inst Perinatol, Dept Infectol & Immunol, Mexico City, DF, Mexico
Sanchez-Serrano, Elias:
Univ Nacl Autonoma Mexico, Chem Fac, Mexico City, DF, Mexico
Loaiza-Flores, Giselle:
Univ Nacl Autonoma Mexico, Chem Fac, Mexico City, DF, Mexico
Plazola-Camacho, Noemi:
Natl Inst Perinatol, Dept Infectol & Immunol, Mexico City, DF, Mexico
Georgina Rodriguez-Delgado, Rosa:
Natl Inst Perinatol, Dept Infectol & Immunol, Mexico City, DF, Mexico
Figueroa-Damian, Ricardo:
Natl Inst Perinatol, Dept Infectol & Immunol, Mexico City, DF, Mexico
Dominguez-Castro, Mauricio:
Natl Inst Perinatol, Dept Physiol & Cellular Dev, Mexico City, DF, Mexico
Lopez-Martinez, Margarita:
Natl Inst Perinatol, Dept Physiol & Cellular Dev, Mexico City, DF, Mexico
Flores-Garcia, Zayra:
Univ Nacl Autonoma Mexico, Fac Higher Educ, Cuautitlan, Mexico State, Mexico
Hernandez-Pineda, Jessica:
Natl Inst Perinatol, Dept Infectol & Immunol, Mexico City, DF, Mexico
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