Silver-pig skin nanocomposites and mesenchymal stem cells: Suitable antibiofilm cellular dressings for wound healing
Por:
Pérez-Díaz M.A., Silva-Bermudez P., Jiménez-López B., Martínez-López V., Melgarejo-Ramírez Y., Brena-Molina A., Ibarra C., Baeza I., Martínez-Pardo M.E., Reyes-Frías M.L., Márquez-Gutiérrez E., Velasquillo C., Martínez-Castañon G., Martinez-Gutierrez F., Sánchez-Sánchez R.
Publicada:
1 ene 2018
Resumen:
Background: Treatment of severe or chronic skin wounds is an important challenge facing medicine and a significant health care burden. Proper wound healing is often affected by bacterial infection; where biofilm formation is one of the main risks and particularly problematic because it confers protection to microorganisms against antibiotics. One avenue to prevent bacterial colonization of wounds is the use of silver nanoparticles (AgNPs); which have proved to be effective against non-multidrug-resistant and multidrug-resistant bacteria. In addition, the use of mesenchymal stem cells (MSC) is an excellent option to improve wound healing due to their capability for differentiation and release of relevant growth factors. Finally, radiosterilized pig skin (RPS) is a biomatrix successfully used as wound dressing to avoid massive water loss, which represents an excellent carrier to deliver MSC into wound beds. Together, AgNPs, RPS and MSC represent a potential dressing to control massive water loss, prevent bacterial infection and enhance skin regeneration; three essential processes for appropriate wound healing with minimum scaring. Results: We synthesized stable 10 nm-diameter spherical AgNPs that showed 21-and 16-fold increase in bacteria growth inhibition (in comparison to antibiotics) against clinical strains Staphylococcus aureus and Stenotrophomonas maltophilia, respectively. RPS samples were impregnated with different AgNPs suspensions to develop RPS-AgNPs nanocomposites with different AgNPs concentrations. Nanocomposites showed inhibition zones, in Kirby-Bauer assay, against both clinical bacteria tested. Nanocomposites also displayed antibiofilm properties against S. aureus and S. maltophilia from RPS samples impregnated with 250 and 1000 ppm AgNPs suspensions, respectively. MSC were isolated from adipose tissue and seeded on nanocomposites; cells survived on nanocomposites impregnated with up to 250 ppm AgNPs suspensions, showing 35% reduction in cell viability, in comparison to cells on RPS. Cells on nanocomposites proliferated with culture days, although the number of MSC on nanocomposites at 24 h of culture was lower than that on RPS. Conclusions: AgNPs with better bactericide activity than antibiotics were synthesized. RPS-AgNPs nanocomposites impregnated with 125 and 250 ppm AgNPs suspensions decreased bacterial growth, decreased biofilm formation and were permissive for survival and proliferation of MSC; constituting promising multi-functional dressings for successful treatment of skin wounds. © 2018 The Author(s).
Filiaciones:
Pérez-Díaz M.A.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Laboratorio de Biomembranas, Prolongacion de Carpio y Plan de Ayala, Col. Santo Tomas, Mexico City, Mexico
Silva-Bermudez P.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Jiménez-López B.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Martínez-López V.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Melgarejo-Ramírez Y.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Brena-Molina A.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Ibarra C.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Baeza I.:
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Laboratorio de Biomembranas, Prolongacion de Carpio y Plan de Ayala, Col. Santo Tomas, Mexico City, Mexico
Martínez-Pardo M.E.:
Instituto Nacional de Investigaciones Nucleares, Banco de Tejidos Radioesterilizados, Carretera México-Toluca S/N La Marquesa, Ocoyoacac, Mexico
Reyes-Frías M.L.:
Instituto Nacional de Investigaciones Nucleares, Banco de Tejidos Radioesterilizados, Carretera México-Toluca S/N La Marquesa, Ocoyoacac, Mexico
Márquez-Gutiérrez E.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Velasquillo C.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
Martínez-Castañon G.:
Universidad Autónoma de San Luis Potosí, Laboratorio de Nanobiomateriales, Facultad de Estomatología, Av. Dr. Manuel Nava No. 2, Zona Universitaria, San Luis Potosí, Mexico
Martinez-Gutierrez F.:
Laboratorio de Microbiología, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava No. 6, Zona Universitaria, San Luis Potosí, Mexico
Sánchez-Sánchez R.:
Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Laboratorio de Biotecnología, Calz. Mexico Xochimilco No 289 Col. Arenal de Guadalupe, Mexico City, Mexico
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