Genetic variability of isolates of Candida parapsilosis complex in two services of a tertiary hospital in Mexico City
Por:
Garcia-Salazar, Eduardo, Duarte-Escalante, Esperanza
Publicada:
1 sep 2017
Categoría:
Medicine (miscellaneous)
Resumen:
The infection caused by the Candida parapsilosis complex may occur
sporadically or in the form of outbreaks, so the study of the genetic
variability in clinical isolates may reveal the presence of endemic
genotypes and the occurrence of horizontal transmission. This study
analyzed the genetic variability of 11 clinical isolates of the C.
parapsilosis complex obtained from Internal Medicine and General Surgery
services of a hospital in Mexico City, using Random Amplification of
Polymorphic DNA (RAPD) with four oligonucleotides (M13, AP3, T3B and
R108), also evaluated whether the patients were infected by the same
genotype. The strain ATCC (R) 22019 T was included in the analysis.
Isolates were identified by VITEK 2 Compact (R) and PCR. With the
polymorphic profiles, a dendrogram was constructed by UPGMA and the
cophenetic correlation coefficient (CCCr), the association index (IA),
and the indicators of genetic diversity were calculated. All isolates
were identified as C. parapsilosis sensu stricto. The dendrogram showed
two groups (I and II). Three genotypes integrated by the strain 22019
and five isolates, mostly associated with invasive candidiasis, were
found in the group I. The group II showed six genotypes composed of six
isolates, mostly associated with mucocutaneous candidiasis. The I A and
the indicators of genetic diversity obtained, revealed a recombinant
reproduction system. The RAPD with the oligonucleotides M13, AP3, T3B
and R108 is useful in the investigation of possible outbreaks caused by
C. parapsilosis and in the determination of their genetic variability.
Filiaciones:
Garcia-Salazar, Eduardo:
Hosp Reg Alta Especialidad Ixtapaluca, Direcc Invest, Pueblo De Zoquiapan 56530, Ixtapaluca, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City, DF, Mexico
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