Population level responses of rotifers (Brachionus calyciflorus and Plationus patulus) to the anti-diabetic drug, metformin
Por:
Garcia-Garcia, G., Reyes-Carrillo, G. I., Sarma, S. S. S., Nandini, S.
Publicada:
1 nov 2017
Resumen:
Aim: Brachionus calyciflorus and Plationus patulus are the two
brachionid rotifers that are widely recognized as suitable bioassay
species for testing the effects of toxicants and xenobiotics. However,
their relative sensitivities vary depending on the duration of exposure,
nature of the toxicants and the ecological tools used. Most
ecotoxicological works on rotifers have considered one species at a time
and mainly tested the effects of pesticides and heavy metals. We
quantified the population level changes in the rotifers B. calyciflorus
and P patulus subjected to different concentrations of metformin, a
widely used anti-diabetic drug.
Methodology: B. calyciflorus and P patulus were separately cultured
starting with a single parthenogenetic individual. The single-celled
green alga, Scenedesmus acutus was used as diet for both the species of
rotifers. The experiments were separately conducted using 25 ml of test
medium with one of the five concentrations of analytical grade metformin
(0 (control). 25, 50, 100 and 200 mu g l(-1). The initial density of
each rotifer was 1 ind.ml(-1). Daily we quantified the number of
rotifers living in each jar and then transferred the surviving
individuals to fresh jars containing appropriate metformin-alga
combination. The experiments were terminated after 16 days.
Results: Peak population abundance and the rate of population increase
(r) of both the rotifers were adversely affected due to metformin. The r
in controls of B. calyciflorus and P patulus were 0.38 +/- 0.02 and 0.26
+/- 0.01 per day, respectively. ForB. calyciflorus and P patulus the r
decreased to 0.19 +/- 0.01 and 0.12 +/- 0.02 per day, respectively, when
exposed to 200 mu r l(-1) of metformin.
Interpretation: The environmental levels of metformin in effluents are
similar to those used in this work. Therefore, metformin should be
considered as harmful for rotifers on long term exposure at
concentrations of as low as 25 mu g l(-1).
Filiaciones:
Garcia-Garcia, G.:
Univ Nacl Autonoma Mexico, Lab Aquat Zool, Campus Iztacala,Av Barrios 1,AP 314, Tlalnepantla 54090, State Of Mexico, Mexico
Reyes-Carrillo, G. I.:
Univ Nacl Autonoma Mexico, Lab Aquat Zool, Campus Iztacala,Av Barrios 1,AP 314, Tlalnepantla 54090, State Of Mexico, Mexico
Sarma, S. S. S.:
Univ Nacl Autonoma Mexico, Lab Aquat Zool, Campus Iztacala,Av Barrios 1,AP 314, Tlalnepantla 54090, State Of Mexico, Mexico
Nandini, S.:
Univ Nacl Autonoma Mexico, Lab Aquat Zool, Campus Iztacala,Av Barrios 1,AP 314, Tlalnepantla 54090, State Of Mexico, Mexico
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