Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Por:
Titaux-Delgado, Gustavo, Carrillo, Elisa, Mendoza, Angeles, Mayorga-Flores, Marlen, Escobedo-Gonzalez, Fatima C., Cano-Sanchez, Patricia, Lopez-Vera, Estuardo, Corzo, Gerardo, del Rio-Portilla, Federico
Publicada:
1 mar 2018
Resumen:
The need for molecules with high specificity against noxious insects
leads the search towards spider venoms that have evolved highly
selective toxins for insect preys. In this respect, spiders as a highly
diversified group of almost exclusive insect predators appear to possess
infinite potential for the discovery of novel insect-selective toxins.
In 2003, a group of toxins was isolated from the spider Macrothele gigas
and the amino acid sequence was reported. We obtained, by molecular
biology techniques in a heterologous system, one of these toxins.
Purification process was optimized by chromatographic methods to
determine the three-dimensional structure by nuclear magnetic resonance
in solution, and, finally, their biological activity was tested. rMagi3
resulted to be a specific insect toxin with no effect on mice.
Filiaciones:
Departamento de Química de Biomacromoléculas, Instituto de QuímicaUniversidad Nacional Autónoma de México, CUCiudad de México04510 México
Laboratorio de Toxinología Marina, Unidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad UniversitariaCiudad de México04510 México
División de Ciencias e Ingenierías, Campus León, Universidad de Guanajuato, Loma del Bosque 103, Col. Lomas del Campestre LeónGto37150 México
Departamento de Medicina Molecular y Bioprocesos, Instituto de BiotecnologíaUniversidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, CuernavacaMorelos61500 México
Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Biomacromol, CU, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Toxinol Marina, Unidad Acad Ecol & Biodiversidad Acuat, Inst Ciencias Mar & Limnol, Ciudad Univ, Mexico City 04510, DF, Mexico
Univ Guanajuato, Div Ciencias & Ingn, Campus Leon,Loma del Bosque 103, Col Lomas Del Campestre 37150, Gto, Mexico
UNAM, Inst Biotecnol, Dept Med Mol & Bioproc, Apartado Postal 510-3, Cuernavaca 61500, Morelos, Mexico
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