Fluorescence Spectroscopy as a Tool for the Assessment of Liver Samples with Several Stages of Fibrosis
Por:
Fabila-Bustos D.A., Arroyo-Camarena U.D., López-Vancell M.D., Durán-Padilla M.A., Azuceno-García I., Stolik-Isakina S., Valor-Reed A., Ibarra-Coronado E., Hernández-Quintanar L.F., Escobedo G., De La Rosa-Vázquez J.M.
Publicada:
1 mar 2018
Resumen:
During the last years, fluorescence spectroscopy has been used as a
potential tool for the evaluation and characterization of tissues with
different disease conditions due to its low cost, high sensitivity, and
minimally or noninvasive character. In this study, fluorescence
spectroscopy was used to study 19 paraffin blocks containing human liver
tissue from biopsies. All samples were previously analyzed by two senior
pathologists in a single-blind trial. After their evaluation, four liver
samples were classified as nonfibrosis (F0), four as initial fibrosis
(F1-F2), four as advanced fibrosis (F3), and six as cirrhosis (F4). The
fluorescence was induced at different wavelengths as follows: 330, 365,
and 405nm using a portable fiber-optic system. The fluorescence spectra
were recorded in the range of 400-750nm. A distinctive correlation
between the shape of each spectrum and the level of fibrosis in the
liver sample was detected. A multi-variate statistical analysis based on
principal component analysis followed by linear discrimination analysis
was applied to develop algorithms able to distinguish different stages
of fibrosis based on the characteristics of fluorescence spectra.
Pairwise comparisons were performed: F0 versus F1-F2, F1-F2 versus F3,
F3 versus F4, and F1-F2 versus F4. The algorithms applied to each set of
data yielded values of sensitivity and specificity that were higher than
90% and 95%, respectively, in all the analyzed cases. With this study,
it is concluded that fluorescence spectroscopy can be used as a
complementary tool for the assessment of liver fibrosis in liver tissue
samples, which sets the stage for subsequent clinical trials.
Filiaciones:
Fabila-Bustos D.A.:
Inst Politecn Nacl, UPIIH, Lab Espect, Ciudad Conocimiento & Cultura, San Agustin Tlaxiaca, Hidalgo, Mexico
Inst Politecn Nacl, ESIME Zac, Lab Biofoton, Mexico City 07738, DF, Mexico
Laboratorio de Espectroscopía, UPIIH, Ciudad Del Conocimiento y la Cultura, Instituto Politécnico Nacional, San Agustín Tlaxiaca, Hidalgo, Mexico
Laboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico, 07738, Mexico
Arroyo-Camarena U.D.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Fac Med, Unidad Med Expt, Mexico City, DF, Mexico
Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Méxic, Hospital General de México dr. Eduardo Liceaga, Ciudad de México, Mexico
López-Vancell M.D.:
Hosp Gen Mexico Dr Eduardo Liceaga, Serv Patol, Mexico City, DF, Mexico
Servicio de Patología, Hospital General de México Dr. Eduardo Liceaga, Ciudad de México, Mexico
Durán-Padilla M.A.:
Hosp Gen Mexico Dr Eduardo Liceaga, Serv Patol, Mexico City, DF, Mexico
Servicio de Patología, Hospital General de México Dr. Eduardo Liceaga, Ciudad de México, Mexico
Azuceno-García I.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Fac Med, Unidad Med Expt, Mexico City, DF, Mexico
Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Méxic, Hospital General de México dr. Eduardo Liceaga, Ciudad de México, Mexico
Stolik-Isakina S.:
Inst Politecn Nacl, ESIME Zac, Lab Biofoton, Mexico City 07738, DF, Mexico
Laboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico, 07738, Mexico
Valor-Reed A.:
Inst Politecn Nacl, ESIME Zac, Lab Biofoton, Mexico City 07738, DF, Mexico
Laboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico, 07738, Mexico
Ibarra-Coronado E.:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Ciudad Univ, Mexico City, DF, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Ciudad Universitaria, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Hernández-Quintanar L.F.:
Inst Politecn Nacl, ESIME Zac, Lab Biofoton, Mexico City 07738, DF, Mexico
Univ Tecnol Mexico, UNITEC MEXICO, Campus Sur, Mexico City, DF, Mexico
Laboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico, 07738, Mexico
Universidad Tecnológica de México-UNITEC Mexico - Campus sur, Ciudad de México, Mexico
Escobedo G.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Fac Med, Unidad Med Expt, Mexico City, DF, Mexico
Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Méxic, Hospital General de México dr. Eduardo Liceaga, Ciudad de México, Mexico
De La Rosa-Vázquez J.M.:
Inst Politecn Nacl, ESIME Zac, Lab Biofoton, Mexico City 07738, DF, Mexico
Laboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico, 07738, Mexico
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