Lipid metabolism and pro-oxidant/antioxidant balance of Halamphora oceanica from the Gulf of Mexico exposed to water accommodated fraction of Maya crude oil
Por:
Olivares-Rubio, Hugo F., Salazar-Coria, Lucia, Najera-Martinez, Minerva, Luis Godinez-Ortega, Jose, Vega-Lopez, Armando
Publicada:
1 ene 2018
Resumen:
Diatoms play key roles in primary production and carbon fixation at a
global scale and in some cases these species live on marine ecosystems
impacted by crude oil (CO) spills. Halamphora oceanica, a new diatom
species from the Southwest of the Gulf of Mexico was isolated and
cultured in the laboratory and was exposed to water accommodated
fraction (WAF) of different Maya CO loads at 0.01, 0.1, 1 and 10 g/L by
96 h. A battery of biomarkers involved in oxidative stress (O-2 center
dot, H2O2, TSARS, ROOH, RC = O, SOD, CAT, GPx), biotransformation and
conjugation (total CYP450 activity and GST) moreover fatty acid (FA)
metabolism (FA levels, fatty-acid synthase and acyl-CoA oxidase) were
measured. Obtained results suggest that increases of PAHs in the medium
(below to EC50) acts as external forces able to turn-on regulatory
mechanisms on H. oceanica involved in both, on the PAHs uptake and
changing its aerobic metabolism to anaerobic metabolism. However, the
growth of this microalgae species evaluated as chlorophyll ``a'' and
pheophytin levels increased as the WAF concentration indicating that
PAHs and other hydrosoluble hydrocarbons were used as carbon and energy
sources by unidentified enzymes not evaluated in the current study. Our
hypothesis was also corroborated by IBRv2. In the current study, we
suppose the change from aerobic to anaerobic metabolism as a strategy
for Halamphora oceanica survival exposed to petroleum hydrocarbons.
Filiaciones:
Olivares-Rubio, Hugo F.:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Lab Toxicol Ambiental, Unidad Profes Zacatenco, Av Wilfrido Massieu S-N, Mexico City 07738, DF, Mexico
Salazar-Coria, Lucia:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Lab Toxicol Ambiental, Unidad Profes Zacatenco, Av Wilfrido Massieu S-N, Mexico City 07738, DF, Mexico
Najera-Martinez, Minerva:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Lab Toxicol Ambiental, Unidad Profes Zacatenco, Av Wilfrido Massieu S-N, Mexico City 07738, DF, Mexico
Luis Godinez-Ortega, Jose:
Univ Nacl Autonoma Mexico, Inst Biol, Apdo Postal 70-233, Mexico City 04510, DF, Mexico
Vega-Lopez, Armando:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Lab Toxicol Ambiental, Unidad Profes Zacatenco, Av Wilfrido Massieu S-N, Mexico City 07738, DF, Mexico
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