Experimental and theoretical approaches to investigate the immunogenicity of Taenia soliumderived KE7 antigen
Por:
Bobes, Raul J., Navarrete-Perea, Jose, Ochoa-Leyva, Adrian, Hugo Anaya, Victor, Hernandez, Marisela, Cervantes-Torres, Jacquelynne, Estrada, Karel, Sanchez-Lopez, Filiberto, Soberon, Xavier, Rosas, Gabriela, Maroni Nunes, Caris, Garcia-Varela, Martin, Rafael Sotelo-Mundo, Rogerio, Alexis Lopez-Zavala, Alonso, Gevorkian, Goar, Acero, Gonzalo, Laclette, Juan P., Fragoso, Gladis, Sciutto, Edda
Publicada:
1 dic 2017
Resumen:
Taenia solium cysticercosis, a parasitic disease that affects human health in various regions of the world, is preventable by vaccination. Both the 97-aminoacid- long KETc7 peptide and its carboxyl-terminal, 18-amino-acid-long sequence (GK-1) are found in Taenia crassiceps. Both peptides have proven protective capacity against cysticercosis and are part of the highly conserved, cestode-native, 264- amino-acid long protein KE7. KE7 belongs to a ubiquitously distributed family of proteins associated with membrane processes and may participate in several vital cell pathways. The aim of this study was to identify the T. solium KE7 (TsKE7) fulllength protein and to determine its immunogenic properties. Recombinant TsKE7 (rTsKE7) was expressed in Escherichia coli Rosetta2 cells and used to obtain mouse polyclonal antibodies. Anti-rTsKE7 antibodies detected the expected native protein among the 350 spots developed from T. solium cyst vesicular fluid in a mass spectrometry-coupled immune proteomic analysis. These antibodies were then used to screen a phage-displayed 7-random-peptide library to map B-cell epitopes. The recognized phages displayed 9 peptides, with the consensus motif Y(F/Y)PS sequence, which includes YYYPS (named GK-1M, for being a GK-1 mimotope), exactly matching a part of GK-1. GK-1M was recognized by 58% of serum samples from cysticercotic pigs with 100% specificity but induced weak protection against murine cysticercosis. In silico analysis revealed a universal T-cell epitope(s) in native TsKE7 potentially capable of stimulating cytotoxic T lymphocytes and helper T lymphocytes under different major histocompatibility complex class I and class II mouse haplotypes. Altogether, these results provide a rationale for the efficacy of the KETc7, rTsKE7, and GK-1 peptides as vaccines. © 2017 American Society for Microbiology.
Filiaciones:
Bobes, Raul J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Navarrete-Perea, Jose:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Inst Nacl Med Genom, Mexico City, DF, Mexico
Ochoa-Leyva, Adrian:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Hugo Anaya, Victor:
Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Morelia, Morelia, Michoacan, Mexico
Hernandez, Marisela:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Cervantes-Torres, Jacquelynne:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Estrada, Karel:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Sanchez-Lopez, Filiberto:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Soberon, Xavier:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Inst Nacl Med Genom, Mexico City, DF, Mexico
Rosas, Gabriela:
Univ Autonoma Estado Morelos, Fac Med, Cuernavaca, Morelos, Mexico
Maroni Nunes, Caris:
Univ Estadual Paulista, UNESP, Dept Anim Hlth & Prod, Aracatuba, SP, Brazil
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Inst Biol, Mexico City, DF, Mexico
Rafael Sotelo-Mundo, Rogerio:
Ctr Invest Alimentac & Desarrollo AC CIAD, Lab Estruct Biomol, Hermosillo, Sonora, Mexico
Alexis Lopez-Zavala, Alonso:
Ctr Invest Alimentac & Desarrollo AC CIAD, Lab Estruct Biomol, Hermosillo, Sonora, Mexico
Univ Sonora, Dept Ciencias Quim Biol, Hermosillo, Sonora, Mexico
Gevorkian, Goar:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Acero, Gonzalo:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Laclette, Juan P.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Fragoso, Gladis:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Sciutto, Edda:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
|