Orally administered Taenia solium Calreticulin prevents experimental intestinal inflammation and is associated with a type 2 immune response
Por:
Mendlovic, Fela, Cruz-Rivera, Mayra, Alfredo Diaz-Gandarilla, Jose, Antonio Flores-Torres, Marco, Avila, Guillermina, Perfiliev, Maria, Maria Salazar, Ana, Arriaga-Pizano, Lourdes, Ostrosky-Wegman, Patricia, Flisser, Ana
Publicada:
16 oct 2017
Resumen:
Intestinal helminth antigens are inducers of type 2 responses and can elicit regulatory immune responses, resulting in dampened inflammation. Several platyhelminth proteins with anti-inflammatory activity have been reported. We have identified, cloned and expressed the Taenia solium calreticulin (rTsCRT) and shown that it predominantly induces a type 2 response characterized by IgG1, IL-4 and IL-5 production in mice. Here, we report the rTsCRT anti-inflammatory activity in a well-known experimental colitis murine model. Mice were orally immunized with purified rTsCRT and colitis was induced with trinitroben-zene sulfonic acid (TNBS). Clinical signs of disease, macroscopic and microscopic tissue inflammation, cytokine production and micronuclei formation, as a marker of genotoxicity, were measured in order to assess the effect of rTsCRT immunization on experimentally induced colitis. rTsCRT administration prior to TNBS instillation significantly reduced the inflammatory parameters, including the acute phase cytokines TNF-a, IL-1ß and IL-6. Dampened inflammation was associated with increased local expression of IL-13 and systemic IL-10 and TGF-ß production. Genotoxic damage produced by the inflammatory response was also precluded. Our results show that oral treatment with rTsCRT prevents excessive TNBS-induced inflammation in mice and suggest that rTsCRT has immunomodulatory properties associated with the expression of type 2 and regulatory cytokines commonly observed in other helminths. © 2017 Mendlovic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Filiaciones:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Univ Anahuac Mexico Norte, Fac Ciencias Salud, Huixquilucan, Estado De Mexic, Mexico
Univ Juarez Autonoma Tabasco, Div Acad Multidisciplinaria Comalcalco, Comalcalco, Tabasco, Mexico
Univ Nacl Autonoma Mexico, Dept Med Genom & Toxicol Ambiental, Inst Invest Biomed, Mexico City, DF, Mexico
Hosp Especialidades CMN Siglo XXI, IMSS, Unidad Invest Med Inmunoquim, Mexico City, DF, Mexico
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