Quantitative multiplexed proteomics of Taenia solium cysts obtained from the skeletal muscle and central nervous system of pigs
Por:
Navarrete-Perea J., Isasa M., Paulo J.A., Corral-Corral R., Flores-Bautista J., Hernández-Téllez B., Bobes R.J., Fragoso G., Sciutto E., Soberón X., Gygi S.P., Laclette J.P.
Publicada:
1 sep 2017
Resumen:
In human and porcine cysticercosis caused by the tapeworm Taenia solium,
the larval stage (cysts) can infest several tissues including the
central nervous system (CNS) and the skeletal muscles (SM). The cyst's
proteomics changes associated with the tissue localization in the host
tissues have been poorly studied. Quantitative multiplexed proteomics
has the power to evaluate global proteome changes in response to
different conditions. Here, using a TMT-multiplexed strategy we
identified and quantified over 4,200 proteins in cysts obtained from the
SM and CNS of pigs, of which 891 were host proteins. To our knowledge,
this is the most extensive intermixing of host and parasite proteins
reported for tapeworm infections. Several antigens in cysticercosis, i.
e., GP50, paramyosin and a calcium-binding protein were enriched in
skeletal muscle cysts. Our results suggested the occurrence of
tissue-enriched antigen that could be useful in the improvement of the
immunodiagnosis for cysticercosis. Using several algorithms for epitope
detection, we selected 42 highly antigenic proteins enriched for each
tissue localization of the cysts. Taking into account the fold changes
and the antigen/epitope contents, we selected 10 proteins and produced
synthetic peptides from the best epitopes. Nine peptides were recognized
by serum antibodies of cysticercotic pigs, suggesting that those
peptides are antigens. Mixtures of peptides derived from SM and CNS
cysts yielded better results than mixtures of peptides derived from a
single tissue location, however the identification of the ` optimal'
tissue-enriched antigens remains to be discovered. Through machine
learning technologies, we determined that a reliable immunodiagnostic
test for porcine cysticercosis required at least five different
antigenic determinants.
Filiaciones:
Navarrete-Perea J.:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City, DF, Mexico
Harvard Med Sch, Dept Cell Biol, Boston, MA USA
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Dept. of Cell Biology, Harvard Medical School, Boston, MA, United States
Isasa M.:
Harvard Med Sch, Dept Cell Biol, Boston, MA USA
Dept. of Cell Biology, Harvard Medical School, Boston, MA, United States
Paulo J.A.:
Harvard Med Sch, Dept Cell Biol, Boston, MA USA
Dept. of Cell Biology, Harvard Medical School, Boston, MA, United States
Corral-Corral R.:
Univ Nacl Autonoma Mexico, Dept Biochem & Struct Biol, Inst Cell Physiol, Mexico City, DF, Mexico
Dept. of Biochemistry and Structural Biology, Institute of Cell Physiology, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Flores-Bautista J.:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City, DF, Mexico
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Hernández-Téllez B.:
Univ Nacl Autonoma Mexico, Sch Med, Dept Tissue & Cell Biol, Mexico City, DF, Mexico
Dept. of Tissue and Cell Biology, School of Medicine, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Bobes R.J.:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City, DF, Mexico
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Fragoso G.:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City, DF, Mexico
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Sciutto E.:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City, DF, Mexico
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Soberón X.:
Inst Nacl Med Genom, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biocatalysis & Cellular Engn, Cuernavaca, Morelos, Mexico
Instituto Nacional de Medicina Genómica, Ciudad de Mexico, Mexico
Dept. of Biocatalysis and Cellular Engineering, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Mexico
Gygi S.P.:
Harvard Med Sch, Dept Cell Biol, Boston, MA USA
Dept. of Cell Biology, Harvard Medical School, Boston, MA, United States
Laclette J.P.:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City, DF, Mexico
Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
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