Whole genome sequencing of Mycobacterium bovis to obtain molecular fingerprints in human and cattle isolates from Baja California, Mexico
Por:
Estrella Sandoval-Azuara, Sarai, Muniz-Salazar, Raquel, Perea-Jacobo, Ricardo, Robbe-Austerman, Suelee, Perera-Ortiz, Alejandro, Lopez-Valencia, Gilberto, Bravo, Doris M., Sanchez-Flores, Alejandro, Miranda-Guzman, Daniela, Alberto Flores-Lopez, Carlos, Zenteno-Cuevas, Roberto, Laniado-Laborin, Rafael, Lafarga de la Cruz, Fabiola, Stuber, Tod P.
Publicada:
1 oct 2017
Resumen:
Objectives To determine genetic diversity by comparing the whole genome sequences of cattle and human Mycobacterium bovis isolates from Baja California. Methods A whole genome sequencing strategy was used to obtain the molecular fingerprints of 172 isolates of M. bovis obtained from Baja California, Mexico; 155 isolates were from cattle and 17 isolates were from humans. Spoligotypes were characterized in silico and single nucleotide polymorphism (SNP) differences between the isolates were evaluated. Results A total of 12 M. bovis spoligotype patterns were identified in cattle and humans. Two predominant spoligotypes patterns were seen in both cattle and humans: SB0145 and SB1040. The SB0145 spoligotype represented 59% of cattle isolates (n = 91) and 65% of human isolates (n = 11), while the SB1040 spoligotype represented 30% of cattle isolates (n = 47) and 30% of human isolates (n = 5). When evaluating SNP differences, the human isolates were intimately intertwined with the cattle isolates. Conclusions All isolates from humans had spoligotype patterns that matched those observed in the cattle isolates, and all human isolates shared common ancestors with cattle in Baja California based on SNP analysis. This suggests that most human tuberculosis caused by M. bovis in Baja California is derived from M. bovis circulating in Baja California cattle. These results reinforce the importance of bovine tuberculosis surveillance and control in this region. © 2017 The Authors
Filiaciones:
Estrella Sandoval-Azuara, Sarai:
Univ Autonoma Baja California, Lab Epidemiol & Ecol Mol, Escuela Ciencias Salud, Blvd Zertuche & Blvd Lagos, Ensenada 22890, Baja California, Mexico
Univ Autonoma Baja California, Fac Ciencias Marinas, Tijuana Ensenada Rd 103 Km, Ensenada 22860, Baja California, Mexico
Muniz-Salazar, Raquel:
Univ Autonoma Baja California, Lab Epidemiol & Ecol Mol, Escuela Ciencias Salud, Blvd Zertuche & Blvd Lagos, Ensenada 22890, Baja California, Mexico
Red Multidisciplinaria Invest TB, Ensenada, Baja California, Mexico
Perea-Jacobo, Ricardo:
Univ Autonoma Baja California, Lab Epidemiol & Ecol Mol, Escuela Ciencias Salud, Blvd Zertuche & Blvd Lagos, Ensenada 22890, Baja California, Mexico
Univ Autonoma Baja California, Fac Ciencias Marinas, Tijuana Ensenada Rd 103 Km, Ensenada 22860, Baja California, Mexico
Robbe-Austerman, Suelee:
USDA, Natl Vet Serv Labs, 1015 N Univ Blvd, Ames, IA 50011 USA
Perera-Ortiz, Alejandro:
Anim & Plant Hlth Inspect Serv, United States Dept Agr, Mexico City, DF, Mexico
Lopez-Valencia, Gilberto:
Univ Autonoma Baja California, Inst Invest Ciencias Vet, Lab TB & Brucelosis, San Felipe Rd 3-5 Km, Mexicali 21386, Baja California, Mexico
Red Multidisciplinaria Invest TB, Ensenada, Baja California, Mexico
Bravo, Doris M.:
USDA, Natl Vet Serv Labs, 1015 N Univ Blvd, Ames, IA 50011 USA
Sanchez-Flores, Alejandro:
UNAM, Inst Biotecnol, Unidad Univ Secuenciac Mas & Bioinformat, Av Univ 2001, Cuernavaca 62210, Morelos, Mexico
Red Multidisciplinaria Invest TB, Ensenada, Baja California, Mexico
Miranda-Guzman, Daniela:
Univ Autonoma Baja California, Inst Invest Ciencias Vet, Lab TB & Brucelosis, San Felipe Rd 3-5 Km, Mexicali 21386, Baja California, Mexico
Alberto Flores-Lopez, Carlos:
Univ Autonoma Baja California, Fac Ciencias, Tijuana Ensenada Rd 103 Km, Ensenada 22860, Baja California, Mexico
Red Multidisciplinaria Invest TB, Ensenada, Baja California, Mexico
Zenteno-Cuevas, Roberto:
Univ Veracruzana, Inst Salud Publ, Xalapa Enriquez 91190, Veracruz, Mexico
Red Multidisciplinaria Invest TB, Ensenada, Baja California, Mexico
Laniado-Laborin, Rafael:
ISESALUD, Hosp Gen Tijuana, Clin TB, Av Centenario 10851, Tijuana 22680, Baja California, Mexico
Red Multidisciplinaria Invest TB, Ensenada, Baja California, Mexico
Lafarga de la Cruz, Fabiola:
Ctr Invest Cient & Educ Superac Ensenada, Dept Acuicultura, Carretera Ensenada Tijuana 3918, Ensenada 22860, Baja California, Mexico
Stuber, Tod P.:
USDA, Natl Vet Serv Labs, 1015 N Univ Blvd, Ames, IA 50011 USA
|