Filling the gaps in the classification of the Digenea Carus, 1863: systematic position of the Proterodiplostomidae Dubois, 1936 within the superfamily Diplostomoidea Poirier, 1886, inferred from nuclear and mitochondrial DNA sequences
Por:
Ivan Hernandez-Mena, David, Garcia-Varela, Martin, Perez-Ponce de Leon, Gerardo
Publicada:
1 oct 2017
Categoría:
Parasitology
Resumen:
The Diplostomida Olson, Cribb, Tkach, Bray & Littlewood, 2003 is the
less diverse order of the two orders within the subclass Digenea Carus,
1863 and is currently classified into three superfamilies, i.e.
Brachylaimoidea Joyeux & Foley, 1930, Diplostomoidea Poirier, 1886, and
Schistosomatoidea Stiles & Hassall, 1898. Although the
suprageneric-level relationships have been elucidated with the use of
molecular markers, the lack of representation of some groups obscure the
phylogenetic relationships among families, rendering the classification
unstable. Here, we tested the phylogenetic position of the family
Proterodiplostomidae Dubois, 1936 based on partial 28S rDNA and complete
18S rDNA sequences for Crocodilicola pseudostoma (Willemoes-Suhm, 1870),
a crocodile parasite that has been found as a progenetic metacercaria
parasitising the pale catfish Rhamdia guatemalensis (Gunther) in Mexico
and in other siluruforms in the Neotropics. We augmented the
representation of the species, genera and families within the
Diplostomida, including mostly representatives of the superfamily
Diplostomoidea, and assembled a dataset that contains 49 species for the
28S rRNA gene, and 45 species for the 18S rRNA gene. Additionally, we
explored the phylogenetic signal of the mitochondrial gene cox1 in
reconstructing the phylogenetic relationships of selected members of the
superfamily. Our analyses showed that the family Proterodiplostomidae is
the sister taxon to the paraphyletic Diplostomidae Poirier, 1886 and
Strigeidae Railliet, 1919, with Cyathocotylidae Muhling, 1898 +
Brauninidae Wolf, 1903 as their sister group. Analysis of concatenated
18S + 28S sequences revealed the Liolopidae Odhner, 1912 as the basal
group of the superfamily Diplostomoidea, although analyses of
independent datasets showed that the position of this family remains
uncertain. Analysis based on cox1 unequivocally resolved the
Proterodiplostomidae as the sister taxon to the Diplostomidae and
Strigeidae, although the Cyathocotylidae was nested in a different
clade, along with brachylaimoids and schistosomatoids.
Filiaciones:
Ivan Hernandez-Mena, David:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencias Biol, Mexico City, DF, Mexico
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Mexico City, DF, Mexico
Perez-Ponce de Leon, Gerardo:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Mexico City, DF, Mexico
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