Nucleologenesis in Trypanosoma cruzi
Por:
Nepomuceno-Mejia, Tomas, Lara-Martinez, Reyna, Hernandez, Roberto, de Lourdes Segura-Valdez, Maria, Jimenez-Garcia, Luis F.
Publicada:
1 jun 2016
Categoría:
Instrumentation
Resumen:
Nucleolar assembly is a cellular event that requires the synthesis and processing of ribosomal RNA, in addition to the participation of pre-nucleolar bodies (PNBs) at the end of mitosis. In mammals and plants, nucleolar biogenesis has been described in detail, but in unicellular eukaryotes it is a poorly understood process. In this study, we used light and electron microscopy cytochemical techniques to investigate the distribution of nucleolar components in the pathway of nucleolus rebuilding during closed cell division in epimastigotes of Trypanosoma cruzi, the etiologic agent of American trypanosomiasis. Silver impregnation specific for nucleolar organizer regions and an ethylenediaminetetraacetic acid regressive procedure to preferentially stain ribonucleoprotein revealed the conservation and dispersion of nucleolar material throughout the nucleoplasm during cell division. Furthermore, at the end of mitosis, the argyrophilic proteins were concentrated in the nucleolar organizer region. Unexpectedly, accumulation of nucleolar material in the form of PNBs was not visualized. We suggest that formation of the nucleolus in epimastigotes of T. cruzi occurs by a process that does not require the concentration of nucleolar material within intermediate nuclear bodies such as mammalian and plant PNBs. © Microscopy Society of America 2016.
Filiaciones:
Nepomuceno-Mejia, Tomas:
Univ Nacl Autonoma Mexico, Lab Electron Microscopy, Fac Sci, Mexico City 04510, DF, Mexico
CONACYT, Reg Ctr Publ Hlth Res, Tapachula, Chiapas, Mexico
Lara-Martinez, Reyna:
Univ Nacl Autonoma Mexico, Lab Electron Microscopy, Fac Sci, Mexico City 04510, DF, Mexico
Hernandez, Roberto:
Univ Nacl Autonoma Mexico, Biomed Res Inst, Dept Mol Biol & Biotechnol, Mexico City 04510, DF, Mexico
de Lourdes Segura-Valdez, Maria:
Univ Nacl Autonoma Mexico, Lab Electron Microscopy, Fac Sci, Mexico City 04510, DF, Mexico
Jimenez-Garcia, Luis F.:
Univ Nacl Autonoma Mexico, Lab Electron Microscopy, Fac Sci, Mexico City 04510, DF, Mexico
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