Role of Wasp and the small GTPases RhoA, RhoB, and Cdc42 during capacitation and acrosome reaction in spermatozoa of English guinea pigs
Por:
Delgado-Buenrostro, Norma L., Mujica, Adela, Chiquete-Felix, Natalia, Deciga-Alcaraz, Alejandro, Medina-Reyes, Estefany I., Uribe-Carvajal, Salvador, Chirino, Yolanda I.
Publicada:
1 oct 2016
Resumen:
Cytoskeleton remodeling is necessary for capacitation and the acrosome
reaction in spermatozoa. F-actin is located in the acrosome and
equatorial region during capacitation, but is relocated in the
post-acrosomal region during the acrosome reaction in spermatozoa from
bull, rat, mice, and guinea pig. Actin polymerization and relocalization
are generally regulated by small GTPases that activate Wasp protein,
which coordinates with Arp2/3, profilin I, and profilin II to complete
cytoskeletal remodeling. This sequence of events is not completely
described in spermatozoa, though. Therefore, the aim of this study was
to determine if Wasp interacts with small GTPases (RhoA, RhoB, and
Cdc42) and proteins (Arp2/3, profilin I, and profilin II) that
co-localize with F-actin during capacitation and the acrosome reaction
in English guinea pig spermatozoa obtained from the vas deferens. The
spermatozoa were capacitated in calcium-free medium, incubated with an
activator or an inhibitor of GTPases, and then induced to acrosome react
using calcium. The distribution patterns of F-actin were compared to the
patterns of Wasp and its putative interaction partners: Wasp and RhoB,
but not RhoA or Cdc42, localization overlap with F-actin during
capacitation and the acrosome reaction. Activation of small GTPases
localized RhoB to the post-acrosomal region whereas their inhibition
prevented acrosome exocytosis. Arp2/3 and profilin II appear to interact
with Wasp in the post-acrosomal region and flagellum, while profilin I
and Wasp could be found in the equatorial region. Thus, Wasp and F-actin
distribution overlap during capacitation and acrosome reaction, and
small GTPases play an important role in cytoskeleton remodeling during
these processes in spermatozoa. Mol. Reprod. Dev. 83: 927-937, 2016 (c)
2016 Wiley Periodicals, Inc.
Filiaciones:
Univ Nacl Autonoma Mexico, Unidad Biomed UBIMED, Fac Estudios Super Iztacala, Tlalnepantla 54090, Estado De Mexic, Mexico
Univ Nacl Autonoma Mexico, Secc Bioquim & Farmacol Humana, Fac Estudios Super Cuautitlan, Cuautitlan 54743, Estado De Mexic, Mexico
Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biol Celular, Mexico City 07360, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Programa Doctorado Ciencias Biomed, Mexico City 07360, DF, Mexico
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