Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
Por:
Nayelli Sanchez-Carranza, Jessica, Alvarez, Laura, Marquina-Bahena, Silvia, Salas-Vidal, Enrique, Cuevas, Veronica, Jiminez, Elizabeth W., Veloz G., Rafael A., Carraz, Maelle, Gonzalez-Maya, Leticia
Publicada:
1 abr 2017
Resumen:
Caesalpinia coriaria (C. coriaria), also named cascalote, has been known
traditionally in Mexico for having cicatrizing and inflammatory
properties. Phytochemical reports on Caesalpinia species have identified
a high content of phenolic compounds and shown antineoplastic effects
against cancer cells. The aim of this study was to isolate and identify
the active compounds of a water: acetone: ethanol (WAE) extract of C.
coriaria pods and characterize their cytotoxic effect and cell death
induction in different cancer cell lines. The compounds isolated and
identified by chromatography and spectroscopic analysis were
stigmasterol, ethyl gallate and gallic acid. Cytotoxic assays on cancer
cells showed different ranges of activities. A differential effect on
cell cycle progression was observed by flow cytometry. In particular,
ethyl gallate and tannic acid induced G2/M phase cell cycle arrest and
showed interesting effect on microtubule stabilization in Hep3B cells
observed by immunofluorescence. The induction of apoptosis was
characterized by morphological characteristic changes, and was supported
by increases in the ratio of Bax/Bcl-2 expression and activation of
caspase 3/7. This work constitutes the first phytochemical and cytotoxic
study of C. coriaria and showed the action of its phenolic constituents
on cell cycle, cell death and microtubules organization.
Filiaciones:
Nayelli Sanchez-Carranza, Jessica:
Univ Autonoma Estado Morelos, Fac Farm, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
Alvarez, Laura:
Univ Autonoma Estado Morelos, Ctr Invest Quim IICBA, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
Marquina-Bahena, Silvia:
Univ Autonoma Estado Morelos, Ctr Invest Quim IICBA, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
Salas-Vidal, Enrique:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca 62209, Morelos, Mexico
Cuevas, Veronica:
Univ Autonoma Estado Morelos, Fac Farm, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
Jiminez, Elizabeth W.:
Univ Autonoma Estado Morelos, Fac Farm, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
Veloz G., Rafael A.:
Univ Guanajuato, Dept Ingn Agroind, Guanajuato 38000, Mexico
Carraz, Maelle:
Univ Toulouse, IRD, UPS, Pharma Dev UMR152, F-31062 Toulouse, France
Gonzalez-Maya, Leticia:
Univ Autonoma Estado Morelos, Fac Farm, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
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