High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico


Por: de Jesus Uribe-Beltran, Magdalena, Patricia Ahumada-Santos, Yesmi, Paz Diaz-Camacho, Sylvia, Alberto Eslava-Campos, Carlos, Ernesto Reyes-Valenzuela, Jesus, Elena Baez-Flores, Maria, Osuna-Ramirez, Ignacio, Delgado-Vargas, Francisco

Publicada: 1 jul 2017
Resumen:
Purpose. This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E. coli strains. Methodology. A total of 205 E. coli isolates from stool samples were collected from 94 children under 5 years old who were outpatients from two hospitals in the city of Culiacan, Sinaloa, Mexico, during the autumn/winter of 2003/04; their resistance profiles to 19 commercial antimicrobials were investigated using the Kirby-Bauer method. The antibacterial activities of extracts/fractions of fruits (i.e. uvalama, Vitex mollis; ayale, Crescentia alata; and arrayan, Psidium sartorianum) were evaluated using the broth microdilution method. Results. All E. coli isolates were susceptible to amikacin, nitrofurantoin and meropenem, and approximately 96% were resistant to at least one antimicrobial, especially carbenicillin (93.2 %), cefuroxime sodium (53.7 %), ampicillin (40 %) and trimethoprim/sulfamethoxazole (35.1 %). Likewise, the frequency of MDR strains (44.9 %) was high, and no significant association with diarrhoea symptoms was found. Remarkably, all fruit extracts/fractions showed antibacterial activity against some, but not all, MDR isolates. The lowest minimal inhibitory concentration values were for the hexane fraction of arrayan (0.25 mg ml(-1)). Conclusion. A high number of antimicrobial-resistant E. coli (especially to beta-lactams and sulfonamides) and MDR isolates were detected in children under 5 years old, irrespective of diarrhoea symptoms; this is novel information for Culiacan, Sinaloa, Mexico. Moreover, our results showed that the studied fruit extracts/fractions are potential alternative or complementary treatments for MDR E. coli strains.

Filiaciones:
de Jesus Uribe-Beltran, Magdalena:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico

Patricia Ahumada-Santos, Yesmi:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico

Paz Diaz-Camacho, Sylvia:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico

Alberto Eslava-Campos, Carlos:
 Univ Nacl Autonoma Mexico, Sch Med, Ciudad Univ, Mexico City 04510, DF, Mexico

 Hosp Infantil de Mexico Federico Gomez, Hemato Oncol & Res Unit, Lab Bacterial Pathogen, Mexico City 06720, DF, Mexico

Ernesto Reyes-Valenzuela, Jesus:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico

Elena Baez-Flores, Maria:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico

Osuna-Ramirez, Ignacio:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico

Delgado-Vargas, Francisco:
 Autonomous Univ Sinaloa, Sch Chem & Biol Sci, Ciudad Univ, Culiacan, Sinaloa, Mexico
ISSN: 00222615
Editorial
SOC GENERAL MICROBIOLOGY, MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND, Reino Unido
Tipo de documento: Article
Volumen: 66 Número: 7
Páginas: 972-980
WOS Id: 000408243600019
ID de PubMed: 28742000

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