Histological Analysis of Intra-Abdominal Adhesions Treated with Sodium Hyaluronate and Carboxymethylcellulose Gel
Por:
Montalvo-Jave, Eduardo Esteban, Mendoza-Barrera, German Eduardo, Garcia-Pineda, Manuel Alejandro, Limon, Alvaro Rodrigo Jaime, Montalvo-Arenas, Cesar, Rodriguez, Andres Eliu Castell, Jurado, Jesus Tapia
Publicada:
3 mar 2016
Categoría:
Surgery
Resumen:
Purpose: To evaluate macro and microscopically the adhesions developed
after using the anti-adherence compound sodium hyaluronate and
carboxymethylcellulose (SH-CBMC) gel and to determine the volume of the
adhesions using a stereological estimation. Methods: The study was
experimental, random, comparative, and prospective. The subjects of the
study were male Wistar rats divided in three groups (n = 10). Group I
(control) included rats with no peritoneal injury. Group II rats had a
2cm diameter injury created bilaterally in the parietal peritoneum at
3cm from the abdominal midline with electrocautery coated with
physiological solution. Group III rats were given the same injuries and
coated with SH-CBMC gel. All groups were followed up postoperatively for
30days, after which a laparotomy was performed to macroscopically
determine the presence and type of adhesions. Experimental models were
euthanized with anesthetic overdose and biopsies were taken for
histopathological examination and stereological estimate of the volume
of adhesions. Results: Macroscopic adhesions were 20% less prevalent in
Group III compared to Group II, which presented 40% more multiple and
firm adhesions, unlike in Group III, in which they were unique and lax.
There was a statistically significant decrease in the presence and
number of adhesions in rats treated with SH-CBMC gel. Inflammatory
infiltrate was significantly lower in rats treated with SH-CBMC gel, but
there were no differences in connective tissue, fibrosis, and
angiogenesis among groups. There was no statistical difference in the
overall volume of adhesions among the treatment groups. Conclusions:
SH-CBMC gel reduces macroscopic presence and number of adhesions and the
severity of the inflammatory infiltrate.
Filiaciones:
Montalvo-Jave, Eduardo Esteban:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Ciudad Univ,Av Univ 3000, Mexico City 04510, DF, Mexico
Hosp Gen Mexico City, Unidad 304, Serv Cirugia Gen, Ssa, Mexico
Mendoza-Barrera, German Eduardo:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Ciudad Univ,Av Univ 3000, Mexico City 04510, DF, Mexico
Garcia-Pineda, Manuel Alejandro:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Ciudad Univ,Av Univ 3000, Mexico City 04510, DF, Mexico
Limon, Alvaro Rodrigo Jaime:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Ciudad Univ,Av Univ 3000, Mexico City 04510, DF, Mexico
Montalvo-Arenas, Cesar:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Mexico City 04510, DF, Mexico
Rodriguez, Andres Eliu Castell:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Mexico City 04510, DF, Mexico
Jurado, Jesus Tapia:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Ciudad Univ,Av Univ 3000, Mexico City 04510, DF, Mexico
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