Fungal diversity and Aspergillus in hospital environments
Por:
Obed Martinez-Herrera, Erick, Guadalupe Frias-De-Leon, Maria, Duarte-Escalante, Esperanza, del Carmen Calderon-Ezquerro, Maria, del Carmen Jimenez-Martinez, Maria, Acosta-Altamirano, Gustavo, Rivera-Becerril, Facundo, Toriello, Conchita, del Rocio Reyes-Montes, Maria
Publicada:
1 ene 2016
Resumen:
Introduction and objective. Nosocomial invasive fungal infections,
particularly aspergillosis, are an increasing problem in
immunocompromised patients. The presented study evaluates fungal
diversity and the presence of Aspergillus in air samples from two
hospitals.
Materials and methods. Over the course of one year (rainy and dry
seasons), the air was sampled from three areas in two hospitals (1 and
2) using a single-stage Andersen viable particle sampler (Thermo
Scientific, Waltham, MA, USA). The fungi were identified by macro-and
micromorphology, and the number of colony forming units (CFU)/m(3) air
and their richness, abundance, and diversity were determined. Isolates
Aspergillus genus were characterized by their thermotolerance.
Results. The CFU/m(3) air was similar at both hospitals during the two
seasons, but different between the sampled areas. Results showed 10
fungal genera for hospital 1, and 8 for hospital 2. The most abundant
were Penicillium, Cladosporium and Aspergillus. The thermotolerance test
confirmed the identification of A. fumigatus section Fumigati. The
highest growth rate was found in Aspergillus section Nigri.
Conclusion. Determining the fungal diversity in the two hospitals was
important because all the species have the potential to be pathogenic,
especially the section Fumigati.
Filiaciones:
Obed Martinez-Herrera, Erick:
Univ Autonoma Metropolitana Xochimilco, Doctorado Ciencias Biol & Salud, Mexico City, DF, Mexico
Guadalupe Frias-De-Leon, Maria:
Hosp Juarez Mexico, Div Invest, Ave Inst Politecn Nacl, Mexico City, DF, Mexico
Duarte-Escalante, Esperanza:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
del Carmen Calderon-Ezquerro, Maria:
Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Mexico City 04510, DF, Mexico
del Carmen Jimenez-Martinez, Maria:
IAP, Fdn Asistencia Privada Conde Valenciana, Inst Oftalmol, Unidad Invest, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico
Acosta-Altamirano, Gustavo:
Inst Seguridad & Serv Sociales Trabajadores Estad, Mexico City, DF, Mexico
Rivera-Becerril, Facundo:
Univ Autonoma Metropolitana Xochimilco, Dept Hombre & Ambiente, Mexico City, DF, Mexico
Toriello, Conchita:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
del Rocio Reyes-Montes, Maria:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
|