Batch and fed-batch culture of Scenedesmus incrassatulus: Effect over biomass, carotenoid profile and concentration, photosynthetic efficiency and non-photochemical quenching
Por:
Cristobal Garcia-Canedo, Juan, Cristiani-Urbina, Eliseo, Flores-Ortiz, Cesar M., Ponce-Noyola, Teresa, Esparza-Garcia, Fernando, Olivia Canizares-Villanueva, Rosa
Publicada:
1 ene 2016
Categoría:
Agronomy and Crop Science
Resumen:
The microalga Scenedesmus incrassatulus was cultivated in batch and
fed-batch culture modes under three different nitrogen concentrations
(N). Considering that nitrogen limitation results in carotenoid
accumulation, main objectives of this work were to assess carotenoid
concentration and the following fluorescence parameters: maximum
photosynthetic efficiency (Fv/Fm), operational photosynthetic efficiency
(Fq'/Fm') and the non-photochemical quenching (NPQ) at different N
concentrations in the culture media, using batch and fed-batch culture
modes. In all treatments, carotenoid concentration reached maximal level
when N concentration in the medium was almost completely depleted.
Carotenoid concentrations in fed-batch treatments were up to 3 times
higher than batch culture treatments. Maximum dry weight of 4.05 +/-
0.04 g L-1 and cell concentration of 8.24 x 107 cell mL(-1) was obtained
using the higher N concentration in fed-batch, these values were
approximately 3 times higher than the obtained in batch culture. Fv/Fm
showed the highest magnitude when the initial N concentration in the
culture medium was higher, for both culture modes. Results showed that
the fed-batch culture of S. incrassatulus promotes a higher Fv/Fm than
batch culture (up to 0.78). The application of fed-batch culture had the
effect of stabilizing the values of Fv/Fm during feeding period. In all
fed-batch treatments the maximum Fq'/Fm' was detected during the feeding
period, which had maximum values ranging from 0.47-0.58. In fed-batch
culture mode NPQ showed no direct relationship with total carotenoid
concentration. ETR was higher in fed batch than in batch cultivation. In
batch cultivation mode ETR had values ranging from 2 to 56 mu mol
electrons m(-2) s(-1). ETR increased 6 fold in fed-batch cultivation
when irradiance was increased, having values up to 610 aemol electrons
m(-2) s(-1). A good cultivation strategy for biocompound production
could be the use of fed batch cultivation aimed to return nutrients like
nitrogen to its initial condition. (C) 2015 Elsevier B.V. All rights
reserved.
Filiaciones:
Cristobal Garcia-Canedo, Juan:
CINVESTAV IPN, Dept Biotecnol & Bioingn, Av Inst Politecn Nacl 2508, Mexico City 07360, DF, Mexico
Cristiani-Urbina, Eliseo:
ENCB IPN, Dept Bioquim, Prolongac Carpio & Plan Ayala S-N, Mexico City 11340, DF, Mexico
Flores-Ortiz, Cesar M.:
Iztacala UNAM, Fac Estudios Super, Unidad Biotecnol & Prototipos, Ave Barrios 1, Tlalnepantla 54090, Edo De Mexico, Mexico
Ponce-Noyola, Teresa:
CINVESTAV IPN, Dept Biotecnol & Bioingn, Av Inst Politecn Nacl 2508, Mexico City 07360, DF, Mexico
Esparza-Garcia, Fernando:
CINVESTAV IPN, Dept Biotecnol & Bioingn, Av Inst Politecn Nacl 2508, Mexico City 07360, DF, Mexico
Olivia Canizares-Villanueva, Rosa:
CINVESTAV IPN, Dept Biotecnol & Bioingn, Av Inst Politecn Nacl 2508, Mexico City 07360, DF, Mexico
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