Aporphine alkaloid contents increase with moderate nitrogen supply in Annona diversifolia Saff. (Annonaceae) seedlings during diurnal periods
Por:
Agustin Orozco-Castillo, Jose, Cruz-Ortega, Rocio, Martinez-Vazquez, Mariano, Rosa Gonzalez-Esquinca, Alma
Publicada:
1 ene 2016
Resumen:
Aporphine alkaloids are secondary metabolites that are obtained in low
levels from species of the Annonaceae family. Nitrogen addition may
increase the alkaloid content in plants. However, previous studies
published did not consider that nitrogen could change the alkaloid
content throughout the day. We conducted this short-term study to
determine the effects of nitrogen applied throughout the diurnal period
on the aporphine alkaloids via measurements conducted on the roots,
stems and leaves of Annona diversifolia seedlings. The 60-day-old
seedlings were cultured with the addition of three levels of nitrogen
(0, 30 and 60mM), and alkaloid extracts were analysed using
high-performance liquid chromatography. The highest total alkaloid
content was measured in the treatment with moderate nitrogen supply.
Further, the levels of aporphine alkaloids changed significantly in the
first few hours of the diurnal period. We conclude that aporphine
alkaloid content increased with moderate nitrogen supply and exhibited
diurnal variation.
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Filiaciones:
Agustin Orozco-Castillo, Jose:
Univ Ciencias & Artes Chiapas, Inst Ciencias Biol, Lab Fisiol & Quim Vegetal, Tuxtla Gutierrez, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencias Biol, Coyoacan, Mexico
Cruz-Ortega, Rocio:
Univ Nacl Autonoma Mexico, Inst Ecol, Delegacion Coyoacan, Mexico
Martinez-Vazquez, Mariano:
Univ Nacl Autonoma Mexico, Inst Quim, Delegacion Coyoacan, Mexico
Rosa Gonzalez-Esquinca, Alma:
Univ Ciencias & Artes Chiapas, Inst Ciencias Biol, Lab Fisiol & Quim Vegetal, Tuxtla Gutierrez, Mexico
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