An Intracellular Arrangement of Histoplasma capsulaturn Yeast-Aggregates Generates Nuclear Damage to the Cultured Murine Alveolar Macrophages
Por:
Pitangui, Nayla de Souza, Sardi, Janaina de Cassia Orlandi, Voltan, Aline R., dos Santos, Claudia T., da Silva, Julhiany de Fatima, da Silva, Rosangela A. M., Souza, Felipe O., Soares, Christiane P., Rodriguez-Arellanes, Gabriela, Lucia Taylor, Maria, Mendes-Giannini, Maria J. S., Fusco-Almeida, Ana M.
Publicada:
11 ene 2016
Resumen:
Histoplasma capsulatum is responsible for a human systemic mycosis that
primarily affects lung tissue. Macrophages are the major effector cells
in humans that respond to the fungus, and the development of respiratory
disease depends on the ability of Histoplasma yeast cells to survive and
replicate within alveolar macrophages. Therefore, the interaction
between macrophages and H. capsulatum is a decisive step in the yeast
dissemination into host tissues. Although the role played by components
of cell-mediated immunity in the host's defense system and the
mechanisms used by the pathogen to evade the host immune response are
well understood, knowledge regarding the effects induced by H.
capsulatum in host cells at the nuclear level is limited. According to
the present findings, H. capsulaturr yeast cells display a unique
architectural arrangement during the intracellular infection of cultured
murine alveolar macrophages, characterized as a formation of aggregates
that seem to surround the host cell nucleus, resembling a ``crown.''
This extranuclear organization of yeast-aggregates generates damage on
the nucleus of the host cell, producing DNA fragmentation and inducing
apoptosis, even though the yeast cells are not located inside the
nucleus and do not trigger changes in nuclear proteins. The current
study highlights a singular intracellular arrangement of H. capsulaturr
yeast near to the nucleus of infected murine alveolar macrophages that
may contribute to the yeast's persistence under intracellular
conditions, since this fungal pathogen may display different strategies
to prevent elimination by the host's phagocytic mechanisms.
Filiaciones:
Pitangui, Nayla de Souza:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
Sardi, Janaina de Cassia Orlandi:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
Voltan, Aline R.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
dos Santos, Claudia T.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
da Silva, Julhiany de Fatima:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
da Silva, Rosangela A. M.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
Souza, Felipe O.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
Soares, Christiane P.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
Rodriguez-Arellanes, Gabriela:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Lucia Taylor, Maria:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Mendes-Giannini, Maria J. S.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
Fusco-Almeida, Ana M.:
UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, Brazil
|