Biochemical and full genome sequence analyses of clinical Vibrio cholerae isolates in Mexico reveals the presence of novel V. cholerae strains
Por:
Alberto Diaz-Quinonez, Jose, Hernandez-Monroy, Irma, Angelica Montes-Colima, Norma, Asuncion Moreno-Perez, Maria, Guadalupe Galicia-Nicolas, Adriana, Lopez-Martinez, Irma, Ruiz-Matus, Cuitlahuac, Kuri-Morales, Pablo, Maria Ortiz-Alcantara, Joanna, Garces-Ayala, Fabiola, Ernesto Ramirez-Gonzalez, Jose
Publicada:
1 may 2016
Resumen:
The first week of September 2013, the National Epidemiological
Surveillance System identified two cases of cholera in Mexico City. The
cultures of both samples were confirmed as Vibrio cholerae serogroup O1,
serotype Ogawa, biotype El Tor. Initial analyses by PFGE and by
PCR-amplification of the virulence genes, suggested that both strains
were similar, but different from those previously reported in Mexico.
The following week, four more cases were identified in a community in
the state of Hidalgo, located 121 km northeast of Mexico City.
Thereafter a cholera outbreak started in the region of La Huasteca.
Genomic analyses of the four strains obtained in this study confirmed
the presence of Pathogenicity Islands VPI-1 and -2, VSP-1 and -2, and of
the integrative element SXT. The genomic structure of the 4 isolates was
similar to that of V. cholerae strain 2010 EL-1786, identified during
the epidemic in Haiti in 2010. (C) 2016 Institut Pasteur. Published by
Elsevier Masson SAS. All rights reserved.
Filiaciones:
Alberto Diaz-Quinonez, Jose:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Mexico City 04510, DF, Mexico
Hernandez-Monroy, Irma:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Angelica Montes-Colima, Norma:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Asuncion Moreno-Perez, Maria:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Guadalupe Galicia-Nicolas, Adriana:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Lopez-Martinez, Irma:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Ruiz-Matus, Cuitlahuac:
Direcc Gen Epidemiol, Mexico City, DF, Mexico
Kuri-Morales, Pablo:
Subsecretaria Prevenc & Promoc Salud, Mexico City, DF, Mexico
Maria Ortiz-Alcantara, Joanna:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Garces-Ayala, Fabiola:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
Ernesto Ramirez-Gonzalez, Jose:
Inst Diagnost & Referencia Epidemiol Dr Manuel Ma, Mexico City, DF, Mexico
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