Comparative Mitogenomics of Leeches (Annelida: Clitellata): Genome Conservation and Placobdella-Specific trnD Gene Duplication
Por:
Oceguera-Figueroa, Alejandro, Manzano-Marin, Alejandro, Kvist, Sebastian, Moya, Andres, Siddall, Mark E., Latorre, Amparo
Publicada:
13 may 2016
Resumen:
Mitochondrial DNA sequences, often in combination with nuclear markers
and morphological data, are frequently used to unravel the phylogenetic
relationships, population dynamics and biogeographic histories of a
plethora of organisms. The information provided by examining complete
mitochondrial genomes also enables investigation of other evolutionary
events such as gene rearrangements, gene duplication and gene loss.
Despite efforts to generate information to represent most of the
currently recognized groups, some taxa are underrepresented in
mitochondrial genomic databases. One such group is leeches (Annelida:
Hirudinea: Clitellata). Herein, we expand our knowledge concerning leech
mitochondrial makeup including gene arrangement, gene duplication and
the evolution of mitochondrial genomes by adding newly sequenced
mitochondrial genomes for three bloodfeeding species: Haementeria
officinalis, Placobdella lamothei and Placobdella parasitica. With the
inclusion of three new mitochondrial genomes of leeches, a better
understanding of evolution for this organelle within the group is
emerging. We found that gene order and genomic arrangement in the three
new mitochondrial genomes is identical to previously sequenced members
of Clitellata. Interestingly, within Placobdella, we recovered a
genus-specific duplication of the trnD gene located between cox2 and
atp8. We performed phylogenetic analyses using 12 protein-coding genes
and expanded our taxon sampling by including GenBank sequences for 39
taxa; the analyses confirm the monophyletic status of Clitellata, yet
disagree in several respects with other phylogenetic hypotheses based on
morphology and analyses of non-mitochondrial data.
Filiaciones:
Oceguera-Figueroa, Alejandro:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Lab Helmintol, Mexico City 04510, DF, Mexico
Natl Museum Nat Hist, Smithsonian Inst, Dept Invertebrate Zool, Washington, DC 20560 USA
Manzano-Marin, Alejandro:
Univ Valencia, Inst Cavanilles Biodiversitat & Biol Evolut, Catedrat Jose Beltran 2, Valencia 46008, Spain
Kvist, Sebastian:
Royal Ontario Museum, Dept Nat Hist, 100 Queens Pk, Toronto, ON M5S 2C6, Canada
Univ Toronto, Dept Ecol & Evolutionary Biol, 25 Willcocks St, Toronto, ON M5S 3B2, Canada
Moya, Andres:
Univ Valencia, Inst Cavanilles Biodiversitat & Biol Evolut, Catedrat Jose Beltran 2, Valencia 46008, Spain
Fdn Fomento Invest Sanitaria & Biomed Comunidad V, Area Genom & Salud, Ave Catalunya 21, Valencia 46020, Spain
Siddall, Mark E.:
Amer Museum Nat Hist, Sackler Inst Comparat Genom, Cent Pk West & 79th St, New York, NY 10024 USA
Latorre, Amparo:
Univ Valencia, Inst Cavanilles Biodiversitat & Biol Evolut, Catedrat Jose Beltran 2, Valencia 46008, Spain
Fdn Fomento Invest Sanitaria & Biomed Comunidad V, Area Genom & Salud, Ave Catalunya 21, Valencia 46020, Spain
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