A Novel Function of Pet54 in Regulation of Cox1 Synthesis in Saccharomyces cerevisiae Mitochondria
Por:
Pablo Mayorga, Juan, Camacho-Villasana, Yolanda, Shingu-Vazquez, Miguel, Garcia-Villegas, Rodolfo, Zamudio-Ochoa, Angelica, Garcia-Guerrero, Aldo E., Hernandez, Greco, Perez-Martinez, Xochitl
Publicada:
22 abr 2016
Resumen:
Cytochrome c oxidase assembly requires the synthesis of the
mitochondria-encoded core subunits, Cox1, Cox2, and Cox3. In yeast,
Pet54 protein is required to activate translation of the COX3 mRNA and
to process the aI5 beta intron on the COX1 transcript. Here we report a
third, novel function of Pet54 on Cox1 synthesis. We observed that Pet54
is necessary to achieve an efficient Cox1 synthesis. Translation of the
COX1 mRNA is coupled to the assembly of cytochrome c oxidase by a
mechanism that involves Mss51. This protein activates translation of the
COX1 mRNA by acting on the COX1 5'-UTR, and, in addition, it interacts
with the newly synthesized Cox1 protein in high molecular weight
complexes that include the factors Coa3 and Cox14. Deletion of Pet54
decreased Cox1 synthesis, and, in contrast to what is commonly observed
for other assembly mutants, double deletion of cox14 or coa3 did not
recover Cox1 synthesis. Our results show that Pet54 is a positive
regulator of Cox1 synthesis that renders Mss51 competent as a
translational activator of the COX1 mRNA and that this role is
independent of the assembly feedback regulatory loop of Cox1 synthesis.
Pet54 may play a role in Mss51 hemylation/conformational change
necessary for translational activity. Moreover, Pet54 physically
interacts with the COX1 mRNA, and this binding was independent of the
presence of Mss51.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet Mol, Mexico City 04510, DF, Mexico
Monash Univ, Sch Biomed Sci, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
Natl Inst Canc INCan, Basic Res Div, Mexico City 14080, DF, Mexico
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