Isolation and characterization of microsatellite markers for Sturnira parvidens and cross-species amplification in Sturnira species
Por:
Gutierrez, Edgar G., Hernandez Canchola, Giovani, Leon Paniagua, Livia S., Martinez Mendez, Norberto, Ortega, Jorge
Publicada:
24 may 2017
Resumen:
Background. Sturnira is one of the most species-rich genera in the
Neotropics, and it is found from Mexico and the Lesser Antilles to
Argentina. This genus forms a well-supported monophyletic clade with at
least twenty-one recognized species, as well as several others under
taxonomic review. Sturnira parvidens is a widespread frugivorous bat of
the deciduous forests of the Neotropics, is highly abundant, and is a
major component in fruit dispersal to regenerate ecosystems.
Methods. We used a technique based on Illumina paired-end sequencing of
a library highly enriched for microsatellite repeats to develop loci for
S. parvidens. We analyzed millions of resulting reads with specialized
software to extract those reads that contained di-, tri-, tetra-,
penta-, and hexanucleotide microsatellites.
Results. We selected and tested 14 polymorphic (di, tri, and tetra)
microsatellites. All markers were genotyped on 26 different individuals
from distinct locations of the distributional area of S. par vi dens. We
observed medium-high genetic variation across most loci, but only 12
were functionally polymorphic. Levels of expected heterozygosity across
all markers were high to medium (mean H-E = 0.79, mean H-O = 0.72). We
examined ascertainment bias in twelve bats of the genus, obtaining
null/monomorphic/polymorphic amplifications.
Discussion. The Illumina paired-end sequencing system is capable of
identifying massive numbers of microsatellite loci, while expending
little time, reducing costs, and providing a large amount of data. The
described polymorphic loci for S. parvidens in particular, and for the
genus in general, could be suitable for further genetic analysis,
including taxonomic inconsistencies, parentage/relatedness analysis, and
population genetics assessments.
Filiaciones:
Gutierrez, Edgar G.:
ENCB, Inst Politecn Nacl, Dept Zool, Mexico City, DF, Mexico
Hernandez Canchola, Giovani:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Evolut, Mexico City, DF, Mexico
Leon Paniagua, Livia S.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Evolut, Mexico City, DF, Mexico
Martinez Mendez, Norberto:
ENCB, Inst Politecn Nacl, Dept Zool, Mexico City, DF, Mexico
Ortega, Jorge:
ENCB, Inst Politecn Nacl, Dept Zool, Mexico City, DF, Mexico
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