Diversity and characterization of airborne bacteria at two health institutions
Por:
Guadalupe Frias-De Leon, Maria, Duarte-Escalante, Esperanza, del Carmen Calderon-Ezquerro, Maria, del Carmen Jimenez-Martinez, Maria, Acosta-Altamirano, Gustavo, Adan Moreno-Eutimio, Mario, Zuniga, Gerardo, Garcia-Gonzalez, Rafael, Ramirez-Perez, Maritona, del Rocio Reyes-Montes, Maria
Publicada:
1 jun 2016
Resumen:
The aim of this study was to identify the types and abundance of
airborne bacteria of two health institutions (1 and 2) and to determine
the genetic association between environmental and clinical isolates of
Staphylococcus spp. Environmental sampling in institutions 1 and 2 was
conducted for 1 year (dry and rainy seasons) using M Air T sampler. The
bacteria and their susceptibility to antibiotics were identified. The
colony-forming units per cubic meter (CFU/m(3)) of air were quantified
for all the isolates, and the diversity and abundance of species were
calculated. The genetic relationship between the clinical and
environmental isolates of S. aureus obtained from institution 1 was
established by the UPGMA based on RAPD markers. At both of the
institutions, the genera most frequently isolated were Staphylococcus
and Bacillus, and the greater concentration of airborne bacteria was
detected during the dry season than the rainy season. The lowest
diversity and highest dominance was found in the institution 2. On the
other hand, at institution 1, the genus that was resistant to
antibiotics was Staphylococcus, whereas at institution 2, no isolate was
found to be resistant to antibiotics. Furthermore, no association
between the clinical and environmental isolates of S. aureus was found.
However, one clone was found in different areas of the institution 1.
The presence of airborne pathogenic bacteria in institutions 1 and 2 is
important to establish the measures for the prevention and control of
nosocomial outbreaks.
Filiaciones:
Guadalupe Frias-De Leon, Maria:
Hosp Juarez Mexico, Div Invest, Av Inst Politecn Nacl 5160, Mexico City 07760, DF, Mexico
Duarte-Escalante, Esperanza:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Col Copilco Univ, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
del Carmen Calderon-Ezquerro, Maria:
Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Col Copilco Univ, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
del Carmen Jimenez-Martinez, Maria:
Fdn Asistencia Privada Conde Valenciana IAP, Inst Oftalmol, Unidad Invest, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Col Copilco Univ, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
Acosta-Altamirano, Gustavo:
Hosp Juarez Mexico, Div Invest, Av Inst Politecn Nacl 5160, Mexico City 07760, DF, Mexico
Adan Moreno-Eutimio, Mario:
Hosp Juarez Mexico, Div Invest, Av Inst Politecn Nacl 5160, Mexico City 07760, DF, Mexico
Zuniga, Gerardo:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Zool, Prol Carpio & Plan Ayala S-N Col Sto Tomas, Mexico City 11340, DF, Mexico
Garcia-Gonzalez, Rafael:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Col Copilco Univ, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
Ramirez-Perez, Maritona:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Col Copilco Univ, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
del Rocio Reyes-Montes, Maria:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Col Copilco Univ, Ciudad Univ 3000, Mexico City 04510, DF, Mexico
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