The use of genetic markers in the molecular epidemiology of histoplasmosis: a systematic review
Por:
Damasceno, L. S., Leitao, T. M. J. S., Taylor, M. L., Muniz, M. M., Zancope-Oliveira, R. M.
Publicada:
1 ene 2016
Resumen:
Histoplasmosis is a systemic mycosis caused by Histoplasma capsulatum, a
dimorphic fungal pathogen that can infect both humans and animals. This
disease has worldwide distribution and affects mainly immunocompromised
individuals. In the environment, H. capsulatum grows as mold but
undergoes a morphologic transition to the yeast morphotype under special
conditions. Molecular techniques are important tools to conduct
epidemiologic investigations for fungal detection, identification of
infection sources, and determination of different fungal genotypes
associated to a particular disease symptom. In this study, we performed
a systematic review in the PubMed database to improve the understanding
about the molecular epidemiology of histoplasmosis. This search was
restricted to English and Spanish articles. We included a combination of
specific keywords: molecular typing [OR] genetic diversity [OR]
polymorphism [AND] H. capsulatum; molecular epidemiology [AND]
histoplasmosis; and molecular epidemiology [AND] Histoplasma. In
addition, we used the specific terms: histoplasmosis [AND] outbreaks.
Non-English or non-Spanish articles, dead links, and duplicate results
were excluded from the review. The results reached show that the main
methods used for molecular typing of H. capsulatum were: restriction
fragment length polymorphism, random amplified polymorphic DNA,
microsatellites polymorphism, sequencing of internal transcribed spacers
region, and multilocus sequence typing. Different genetic profiles were
identified among H. capsulatum isolates, which can be grouped according
to their source, geographical origin, and clinical manifestations.
Filiaciones:
Damasceno, L. S.:
FIOCRUZ Fundacao Oswaldo Cruz, Lab Micol, Setor Imunodiagnost, INI Inst Nacl Infectol Evandro Chagas, BR-21045900 Rio De Janeiro, RJ, Brazil
Leitao, T. M. J. S.:
Univ Fed Ceara, Fac Med, Dept Saude Comunitaria, BR-60430140 Fortaleza, Ceara, Brazil
Taylor, M. L.:
Univ Nacl Autonoma Mexico, Fac Med, Lab Inmunol Hongos, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Muniz, M. M.:
FIOCRUZ Fundacao Oswaldo Cruz, Lab Micol, Setor Imunodiagnost, INI Inst Nacl Infectol Evandro Chagas, BR-21045900 Rio De Janeiro, RJ, Brazil
Zancope-Oliveira, R. M.:
FIOCRUZ Fundacao Oswaldo Cruz, Lab Micol, Setor Imunodiagnost, INI Inst Nacl Infectol Evandro Chagas, BR-21045900 Rio De Janeiro, RJ, Brazil
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