Morphological Studies of Nucleologenesis in Giardia lamblia
Por:
Lara-Martinez, Reyna, De Lourdes Segura-Valdez, Maria, De la Mora-De la Mora, Ignacio, Lopez-Velazquez, Gabriel, Felipe Jimenez-Garcia, Luis
Publicada:
1 may 2016
Resumen:
The nucleolus is a nuclear organelle involved in ribosome biogenesis. In
most eukaryotes this structure disperses during prophase through
anaphase and reorganizes at telophase by a process known as
nucleologenesis. This process involves new transcription of ribosomal
DNA at the nucleolar organizer region and the formation of prenucleolar
bodies fusing to it. In Giardia lamblia, for a long time considered the
only anucleolated eukaryote, a very small nucleolus has been recently
described. In order to evaluate whether nucleologenesis is also present
in Giardia, we analyzed the distribution of nucleolar material during
telophase using different light and electron microscopy techniques
including silver staining for the nucleolar organizer. Results indicate
that in G. lamblia, nucleolar elements persist mainly as an intranuclear
peripheral organelle during all stages of division, including telophase,
however, no prenucleolar bodies are detected in the nucleoplasm.
Therefore, in the parasite, nucleolar material is present throughout
cell division including telophase and formation of prenucleolar bodies
may not be required for nucleologenesis. Anat Rec, 299:549-556, 2016.
(c) 2016 Wiley Periodicals, Inc.
Filiaciones:
Lara-Martinez, Reyna:
Univ Nacl Autonoma Mexico, Fac Sci, Dept Cell Biol, Mexico City, DF, Mexico
De Lourdes Segura-Valdez, Maria:
Univ Nacl Autonoma Mexico, Fac Sci, Dept Cell Biol, Mexico City, DF, Mexico
De la Mora-De la Mora, Ignacio:
Natl Inst Pediat, Lab Genet Biochem, Mexico City, DF, Mexico
Lopez-Velazquez, Gabriel:
Natl Inst Pediat, Lab Genet Biochem, Mexico City, DF, Mexico
Felipe Jimenez-Garcia, Luis:
Univ Nacl Autonoma Mexico, Fac Sci, Dept Cell Biol, Mexico City, DF, Mexico
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