Stress-induced leokocytospermia in rams with healthy reproductive tract
Por:
Isauro Garrido-Farina, German, Castillo-Hernandez, Gabriela, Luis Gutierrez-Hernandez, Jose, Martinez Perez, Diana Isabel, Romero Ramirez, Carlos M., Terrazas Garcia, Angelica, Tortora Perez, Jorge Luis
Publicada:
1 abr 2016
Resumen:
Cytological alterations were inspected in the semen of 24 clinically
healthy rams when subjected to social rearrangement as a stress-inducing
treatment. Rams were lodged in four stalls with six animals each, and
their hierarchical behavior were observed during feeding. The dominance
rates (DR) were established, and rams were classified into dominant (D),
intermediate (Dm) and submissive (S). Blood samples were collected
weekly, and serum cortisol levels determined by radioimmunoassay. Weekly
semen samples were obtained by electroejaculation; cells different from
sperm were counted and sperm anomalies were examined. On week 8, S rams
presented high cortisol levels, D rams showed intermediate levels, and
Dm rams revealed the lowest levels (p < 0.05). The 24 rams showed an
increase in sperm neutrophil count after stress treatment, which reached
its highest levels 13 days after the regrouping; initial values were
recovered on day 24. Exfoliated epithelial cell (EEC) count only showed
significant variations (p < 0.05) in the Dm group, before and after the
stress treatment. Leukocytospermia (LS) and EECs showed inverse
evolution along the experiment. When cortisol levels rose, sperm
anomalies increased significantly (p < 0.05) reaching values between 8
and 18%. Present results indicate that the presence of neutrophils in
ram sperm should be reconsidered in association with stressful events,
and not only as indicative of inflammatory or infectious processes of
the ram reproductive tract. (C) 2016 Elsevier B.V. All rights reserved.
Filiaciones:
Isauro Garrido-Farina, German:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54712, Estado De Mexic, Mexico
Castillo-Hernandez, Gabriela:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54712, Estado De Mexic, Mexico
Luis Gutierrez-Hernandez, Jose:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54712, Estado De Mexic, Mexico
Martinez Perez, Diana Isabel:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54712, Estado De Mexic, Mexico
Romero Ramirez, Carlos M.:
Univ Autonoma Metropolitana, Dept Reprod Biol, Calle La Purisima 186, Mexico City 09310, DF, Mexico
Terrazas Garcia, Angelica:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54712, Estado De Mexic, Mexico
Tortora Perez, Jorge Luis:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54712, Estado De Mexic, Mexico
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