Analysis of the Bacterial Diversity in Liver Abscess: Differences between Pyogenic and Amebic Abscesses
Por:
Reyna-Fabian, Miriam E., Zermeno, Valeria, Ximenez, Cecilia, Flores, Janin, Romero, Miguel F., Diaz, Daniel, Argueta, Jesus, Moran, Patricia, Valadez, Alicia, Cerritos, Rene
Publicada:
1 ene 2016
Resumen:
Several recent studies have demonstrated that virulence in Entamoeba
histolytica is triggered in the presence of both pathogenic and
nonpathogenic bacteria species using in vitro and in vivo experimental
animal models. In this study, we examined samples aspirated from abscess
material obtained from patients who were clinically diagnosed with
amebic liver abscess (ALA) or pyogenic liver abscess (PLA). To determine
the diversity of bacterial species in the abscesses, we performed
partial 16S rRNA gene sequencing. In addition, the E. histolytica and
Entamoeba dispar species were genotyped using tRNA-linked short tandem
repeats as specific molecular markers. The association between clinical
data and bacterial and parasite genotypes were examined through a
correspondence analysis. The results showed the presence of numerous
bacterial groups. These taxonomic groups constitute common members of
the gut microbiota, although all of the detected bacterial species have
a close phylogenetic relationship with bacterial pathogens. Furthermore,
some patients clinically diagnosed with PLA and ALA were coinfected with
E. dispar or E. histolytica, which suggests that the virulence of these
parasites increased in the presence of bacteria. However, no specific
bacterial groups were associated with this effect Together, our results
suggest a nonspecific mechanism of virulence modulation by bacteria in
Entamoeba.
Filiaciones:
Reyna-Fabian, Miriam E.:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Zermeno, Valeria:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Ximenez, Cecilia:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Flores, Janin:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Romero, Miguel F.:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 06726, DF, Mexico
Diaz, Daniel:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 06726, DF, Mexico
Argueta, Jesus:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Moran, Patricia:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Valadez, Alicia:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
Cerritos, Rene:
Univ Nacl Autonoma Mexico, Dept Med Expt, Fac Med, Mexico City 06726, DF, Mexico
|