Alternative energy production pathways in Taenia crassiceps cysticerci in vitro exposed to a benzimidazole derivative (RCB20)
Por:
Fraga, Carolina Miguel, Da Costa, Tatiane Luiza, De Castro, Ana Maria, Reynoso-Ducoing, Olivia, Ambrosio, Javier, Hernandez-Campos, Alicia, Castillo, Rafael, Vinaud, Marina Clare
Publicada:
1 abr 2016
Resumen:
Biochemical studies of benzimidazole derivatives are important to
determine their mode of action and activity against parasites. The lack
of antihelminthic alternatives to treat parasitic infections and
albendazole resistance cases make the search for new antiparasitary
drugs of utmost importance. The
6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole (RCB20)
is a benzimidazole derivative with promising effect. This study
evaluated the effect of different concentrations of RCB20 in the
alternative energetic pathway of in vitro Taenia crassiceps cysticerci.
The parasites were in vitro exposed to 6.5 and 13 mu M of RCB20 and
albendazole sulfoxide (ABZSO). The quantification of acetate,
acetoacetate, beta-hydroxybutyrate, fumarate and propionate was
performed by high-performance liquid chromatography. The quantification
of urea, creatinine and total proteins was performed by
spectrophotometry. The increase in beta-hydroxybutyrate reflects the
enhancement of the fatty acid oxidation in the treated groups. Volatile
fatty acids secretion, acetate and propionate, was increased in the
treated groups. The secretion mechanisms of the treated parasites were
impaired due to organic acids increased concentrations in the
cysticerci. It is possible to conclude that the metabolic effect on
alternative energetic pathways is slightly increased in the parasites
treated with RCB20 than the ones treated with ABZSO.
Filiaciones:
Fraga, Carolina Miguel:
Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Lab Studies Host Parasite Relationship, Rua 235,S-N,Setor Univ, BR-74605050 Goiania, Go, Brazil
Da Costa, Tatiane Luiza:
Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Lab Studies Host Parasite Relationship, Rua 235,S-N,Setor Univ, BR-74605050 Goiania, Go, Brazil
De Castro, Ana Maria:
Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Lab Studies Host Parasite Relationship, Rua 235,S-N,Setor Univ, BR-74605050 Goiania, Go, Brazil
Reynoso-Ducoing, Olivia:
Univ Nacl Autonoma Mexico, Lab Biol Citoesqueleto, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Ambrosio, Javier:
Univ Nacl Autonoma Mexico, Lab Biol Citoesqueleto, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Hernandez-Campos, Alicia:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Mexico City 04510, DF, Mexico
Castillo, Rafael:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Mexico City 04510, DF, Mexico
Vinaud, Marina Clare:
Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Lab Studies Host Parasite Relationship, Rua 235,S-N,Setor Univ, BR-74605050 Goiania, Go, Brazil
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