Sechium edule (Jacq.) Swartz, a New Cultivar with Antiproliferative Potential in a Human Cervical Cancer HeLa Cell Line
Por:
Salazar-Aguilar S., Ruiz-Posadas L.M., Cadena-Iñiguez J., Soto-Hernández M., Santiago-Osorio E., Aguiñiga-Sánchez I., Rivera-Martínez A.R., Aguirre-Medina J.F.
Publicada:
1 ago 2017
Resumen:
The Sechium edule Perla Negra cultivar is a recently-obtained biological
material whose progenitors are S. edule var. nigrum minor and S. edule
var. amarus silvestrys, the latter of which has been reported to have
antiproliferative activity against the HeLa P-388 and L-929 cancer cell
lines. The present study aimed to determine if the methanolic extract of
the fruit of the Perla Negra cultivar had the same biological activity.
The methanolic extract was phytochemically characterized by thin layer
chromatography (TLC) and column chromatography (CC), identifying the
terpenes and flavonoids. The compounds identified via high performance
liquid chromatography (HPLC) were Cucurbitacins B, D, E, and I for the
terpene fractions, and Rutin, Phlorizidin, Myricetin, Quercetin,
Naringenin, Phloretin, Apigenin, and Galangin for the flavonoid
fractions). Biological activity was evaluated with different
concentrations of the methanolic extract in the HeLa cell line and
normal lymphocytes. The methanolic extract inhibited the proliferation
of HeLa cells (IC50 1.85 mu g.mL(-1)), but the lymphocytes were affected
by the extract (IC50 30.04 mu g.mL(-1)). Some fractions, and the pool of
all of them, showed inhibition higher than 80% at a concentration of
2.11 mu g.mL(-1). Therefore, the biological effect shown by the
methanolic extract of the Perla Negra has some specificity in inhibiting
tumor cells and not normal cells; an unusual feature among molecules
investigated as potential biomedical agents.
Filiaciones:
Salazar-Aguilar S.:
Campus Montecillo Km 36.5 Mexico-Texcoco Highway, Texcoco, C.P. 56230, Mexico
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
Ruiz-Posadas L.M.:
Campus Montecillo Km 36.5 Mexico-Texcoco Highway, Texcoco, C.P. 56230, Mexico
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
Cadena-Iñiguez J.:
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
Campus San Luis Potosí, Iturbide No. 73 Street, Salinas de Hidalgo, San Luis Potosí C.P. 78600, Mexico
Soto-Hernández M.:
Campus Montecillo Km 36.5 Mexico-Texcoco Highway, Texcoco, C.P. 56230, Mexico
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
Santiago-Osorio E.:
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
FES Zaragoza, National Autonomous University of Mexico, Batalla 5 de mayo esq. Fuerte de Loreto, Col. Ejército de Oriente, Mexico City, C.P. 09230, Mexico
Aguiñiga-Sánchez I.:
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
FES Zaragoza, National Autonomous University of Mexico, Batalla 5 de mayo esq. Fuerte de Loreto, Col. Ejército de Oriente, Mexico City, C.P. 09230, Mexico
Rivera-Martínez A.R.:
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
FES Zaragoza, National Autonomous University of Mexico, Batalla 5 de mayo esq. Fuerte de Loreto, Col. Ejército de Oriente, Mexico City, C.P. 09230, Mexico
Aguirre-Medina J.F.:
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Agustín Melgar 10 Street, Texcoco, C.P. 56153, Mexico
School of Higher Education of Agricultural Science, Autonomous University of Chiapas, Junction Costera Higway and Pueblo de Huehuetán, Huehuetán, Chiapas C.P. 30660, Mexico
|