Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
Por:
Guzman-Cornejo, Carmen, Garcia-Prieto, Luis, Rebollo-Hernandez, Andrea, Venzal, Jose M., Nava, Santiago, Sanchez-Montes, Sokani
Publicada:
1 jun 2017
Categoría:
Parasitology
Resumen:
Ornithodoros brodyi and Ornithodoros yumatensis are two species
distributed in the Americas and associated with bats and caves. Both
species have similar morphology, and the diagnostic traits of adults
have not been detailed or illustrated accurately. In this study, the
independence of both species is validated on the basis of molecular
evidence (using partial sequences of 16S rDNA gene), and the
morphological differences between them (dentition of the hypostome and
traits of individual mammillae) are confirmed through light and scanning
electron microscopy. In addition to the above characteristics, we
observed other traits that may serve to differentiate both species:
dorsal setae are short and thick in O. yumatensis and are thin and
moderate in size in O. brodyi. We also observed a conspicuous hood in O.
brodyi, which was absent in O. yumatensis. Another characteristic
observed is a line of setae, near the end of Tarsi II-IV, which in O.
brodyi is formed by less than five setae and in O. yumatensis by more
than five. The main morphological difference between larvae of the 2
species is the number of ventral setae [9 (4 circumanal pairs) in O.
brodyi and 8 (3 circumanal pairs) in O. yumatensis]. The genetic
divergence in 16S rDNA sequences between these two species ranges from
9.7 to 10.6%.
Filiaciones:
Univ Nacl Autonoma Mexico, Dept Biol Comparada, Fac Ciencias, Lab Acarol, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Colecc Nacl Helmintos, Mexico City, DF, Mexico
Inst Nacl Tecnol Agr, Rafaela, Santa Fe, Argentina
Consejo Nacl Invest Cient & Tecn, Rafaela, Santa Fe, Argentina
Univ Republ, Lab Vectores & Enfermedades Transmitidas, Fac Vet, CENUR Litoral Norte Salto, Salto, Uruguay
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